Reliable Analytic Strategy to Correlate the Morphological and Cytological Parameters on Lupinus termis L, against Fusarium oxysporum Infection  

作　　者：Ramadan Abd Elghany Mohamed Heba Hassan Elsalahy Osama Abdel-Hafeez Al-Bedak Hoda Abd-EI-Fatah Mostafa Ahmed Nemmat Abd Elgawad Hussein 

机构地区：[1]Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt [2]Mycological Centre, Faculty of Science, Assiut University, Assiut 71516, Egypt [3]Plant Pathology Research Institute, Agriculture Research Center (ARC), Giza 12619, Egypt [4]Faculty of Life Sciences, Department of Agronomy and Crop Science, Humboldt University of Berlin, Albrecht-Thaer-Weg 5,Berlin 14195, Germany

出　　处：《Journal of Agricultural Science and Technology(A)》2015年第7期585-600,共16页农业科学与技术（A）

摘　　要：Many analytic strategies have emerged to estimate plant responses to Fusarium wilt. The demand for fast and reliable method （diagnosis, prediction） to determine isolate strength accurately is not established yet. Early determination of pathogen strength helps in plant medication. The aim of this study was to develop a faster strategy and method for early determination of fungal isolates strength in correlation to plant response. Till now, the scientists have no consensus on the most correlated parameters that could express wilt precisely. In this study, 30 isolates of Fusarium oxysporum isolated from Lupinus termis L. were used to provide an explicit image about the real strength of Fusarium isolates and its impact on the plant. Wilting percentage ranged from 26.67% to 93.33% of the infected plants depending on isolate virulence. Some of cellular, morphological and physical measurements were conducted on 8 out of 30 isolates, including root （length, fresh weight （FW） and dry weight （DW））, nodules （water content （WC）, FW, DW）, stem （height, WC, FW, DW）, total leaves/plant （WC, FW, DW） and the fourth leaf （WC, FW, DW, leaf area, epidermal cell area, epidermal cell number, succulence）. Hierarchical clustering was used to determine the variance between the isolates. Detrended correspondence analysis （DCA） and canonical correspondence analysis （CCA） were used to determine the most important growth parameters that could express wilting accurately. The CCA results showed that most of the measured parameters on the fourth leaf, except for leaf epidermal cell number, were highly and positively correlated to wilt. That makes these specific parameters valuable and sensitive for any changes in isolates strength. Accordingly, a mathematical model was created to be helpful in the quick determination of isolate strength and precise medication.

关 键 词：F. oxysporum L. termis L. leaf area epidermal cell area water content linear and exponential decay models. 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治] S965.122[农业科学—植物保护]