126份番茄材料的抗性基因分子标记检测  

Molecular Marker Detection of Resistant Genes from 126 Tomato Varieties

作　　者：陈宝玲[1] 甘桂云[2] 王先裕[1] 于琴芝 龙安四[1] 

机构地区：[1]广西大学农学院,广西南宁530004 [2]广西农业科学院蔬菜研究所,广西南宁530007 [3]桂林市经济作物技术推广站,广西桂林541001

出　　处：《中国蔬菜》2016年第6期34-41,共8页China Vegetables

基　　金：广西科学研究与技术开发计划项目(桂科攻14121006-5-2;桂科合14123001-3;桂科攻13254002-2);国家大宗蔬菜产业技术体系桂林综合试验站项目(CARS-25-G-37);百色国家农业科技园区建设示范项目(桂科能1598022-1-1)

摘　　要：利用分子标记技术,对56份大、中果型番茄进行烟草花叶病毒病抗性基因Tm-2a、番茄黄化曲叶病毒病抗性基因Ty-1、Ty-2、Ty-3、叶霉病抗性基因Cf-9、晚疫病抗性基因Ph-3、枯萎病抗性基因I-2和根结线虫病抗性基因Mi-1 8个抗性基因的检测,对70份小果型番茄进行Tm-2a、Ty-1、Cf-9、I-2、Mi-1 5个抗性基因的检测。共获得含烟草花叶病毒病抗性基因Tm-2a的材料71份;在番茄黄化曲叶病毒病的3个抗性基因方面,含纯合抗性基因Ty-1的材料7份,杂合16份;含杂合抗性基因Ty-2的材料1份;含纯合抗性基因Ty-3的材料5份,杂合7份。真菌性病害方面,含叶霉病抗性基因Cf-9的材料101份;含纯合晚疫病抗性基因Ph-3的材料1份,杂合6份;含枯萎病抗性基因I-2的材料68份;含根结线虫病抗性基因Mi-1的材料45份。供试番茄材料中,DHG-27同时含有7个抗性基因;含有6个抗性基因的材料有5份,分别为:DHG-11、DHG-20、DHG-34、ZHG-8、HG-4;含有5个抗性基因的有HG-3、XHG-352份材料。Molecular marker technolocy was used to test 8 resistant genes from 56 tomato materials with large and middle fruit types including Tobacco mosaic virus disease resistant gene Tin-2^a, Tomato yellow leaf curl virus disease resistant genes Ty-1, Ty-2, Ty-3, leaf mould resistant gene Cf-9, late blight resistant gene homozygous Ph-3, fusarium wilt resistant genes 1-2, and root knot nematode resistant gene of Mi-1. Besides, 5 resistant genes including Tin-2^a, Ty-1, Cf-9, I-2, Mi-1 from 70 tomato materials of small fruit types were also tested. 71 materials with resistant gene Tm-2^a, 7 materials with pure resistant gene Ty-1, 16 materials with heterozygosis resistant gene Ty-1, 1 material with heterozygosis resistant gene Ty-2, 5 materials with pure resistant gene Ty-3, 7 materials with heterozygosis resistant gene Ty-3, 101 materials with resistant gene Cf-9, 1 material with pure resistant gene Ph-3, 6 materials with heterozygosis resistant gene Ph-3, 68 materials with resistant gene I-2, 45 materials with resistant gene Mi-1.Among them, DHG-27 contains 7 resistant genes. There were 5 materials （ DHG-11, DHG-20, DHG-34, ZHG-8, HG-4 ） containing 6 resistant genes. There were 2 materials （ HG-3 and XHG-35 ） containing 5 resistant genes.

关 键 词：番茄 分子标记 抗性基因检测 

分 类 号：S641.2[农业科学—蔬菜学]