复肾功方对慢性肾功能衰竭大鼠肾脏nephrin mRNA表达的影响  

Effect of Fushengong Decoction on the Expression of Nephrin mRNA in Kidney of Rats with Chronic Renal Failure

作　　者：王玲[1] 黄学宽[1] 万磊[1] 杨玉[1] 

机构地区：[1]重庆医科大学中医药学院,重庆401331

出　　处：《四川大学学报（医学版）》2016年第3期342-346,共5页Journal of Sichuan University(Medical Sciences)

基　　金：重庆市自然科学基金(No.cstc2011jjA10058);重庆市九龙坡区科技计划项目(九龙坡科委发[2011]62号;[2013]37号)资助

摘　　要：目的研究复肾功方对慢性肾功能衰竭(chronic renal failure,CRF)大鼠肾组织足细胞裂孔隔膜蛋白(nephrin)mRNA表达的影响,探讨其降低尿蛋白、减轻肾损害的作用机制。方法将55只雄性SD大鼠随机分为正常组,模型组,复肾功方低、中、高剂量组,每组11只。正常组常规饲养,其余4组大鼠食用含腺嘌呤饲料建立慢性肾功能衰竭模型,连续喂养21d。造模成功后,所有大鼠改用常规饲料。正常组和模型组按20mL/(kg·d)灌胃给予生理盐水;复肾功方低、中、高剂量组按大鼠体质量分别以生药4、8、16g/kg灌胃复肾功方,1次/d,连续灌胃30d。实验结束后,收集大鼠尿液测定24h尿蛋白定量,检测各组大鼠血清血肌酐(SCr)和尿素氮(BUN)水平,HE染色检测肾小球组织形态学改变,免疫荧光检测nephrin蛋白表达,Real-Time PCR法观察肾脏nephrin mRNA表达情况。结果与正常组比较,模型组24h尿蛋白定量、SCr和BUN水平升高(P<0.05),nephrin蛋白及mRNA表达下降(P<0.05);与模型组比较,经复肾功方治疗后,24h尿蛋白定量、SCr和BUN水平降低(P<0.05),肾组织nephrin蛋白及mRNA的表达升高(P<0.05)。结论复肾功方降低CRF大鼠尿蛋白,减轻肾损害程度,其机制可能与升高肾组织nephrin蛋白及mRNA的表达、减轻足细胞损伤有关。Objective To investigate the effect of Fushengong Decoction on the expression of nephrin mRNA in renal tissue of rats with chronic renal failure（CRF）.Methods Fifty five male SD rats were randomly divided into five groups：control group,CRF model group,and low,medium and high Fushengong Decoction dose groups.Rats in control group were fed with standard chow,while the other four groups were fed with adenine to make CRF.The rats in control group received intra-gastric normal saline（NS）of 20 mL/（kg·d）for 30 d,while those in low,medium and high Fushengong Decoction dose groups received Fushengong Decoction at the dose of 4g/kg,8g/kg and 16g/kg respectively,once a day for 30 d.After that,24 hurinary protein in urine was measured,blood urea nitrogen（BUN）and serum creatinine（SCr）were detected.Histomorphology of glomerulus were studied by HE staining,and the expression of nephrin were detected by immunohistochemistry and RT-PCR.Results Compared to the control group,the levels of 24 hurinary protein,BUN and SCr increased significantly（P〈0.05）and the expression of nephrin protein and mRNA decreased significantly（P〈0.05）in CRF model group.The renal interstitium showed fibrotic lesions in model group.The levels of 24 hurinary protein,BUN and SCr decreased significantly after the treatment of Fushengong Decoction（P〈0.05）,while the expression of nephrin protein and mRNA increased significantly（P〈0.05）.Conclusion Fushengong Decoction could reduce urinary protein and relieve renal damage in rats with CRF by improving the expression of nephrin and reducing the injury of podocytes.

关 键 词：复肾功方 慢性肾功能衰竭 足细胞裂孔隔膜蛋白 大鼠 

分 类 号：R285.5[医药卫生—中药学]