Transient Expression of VHB and GLUT1 in the Green Alga Haematococcus pluvialis Using Agrobacteriummediated Transformation  

作　　者：Jinfei YAN Zhaokai WANG Zhiwei YI Runying ZENG 

机构地区：[1]Department of Water Purification, Institute of Seawater Desalination & Multipurpose Utilization, State Oceanic Administration, Tianjin 116001, China [2]Engineering Research Center of Marine Biological Resource Comprehensive Utilization, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China

出　　处：《Agricultural Biotechnology》2016年第2期27-30,33,共5页农业生物技术（英文版）

基　　金：Supported by Project of the National High Technology Research and Development Program("863"Program)of China(Grant No.2012AA092103);Scientific Research Foundation of the Institute of Seawater Desalination&Multipurpose Utilization(Grant No.K-JBYWF-2015-T20);Project of the Third Institute of Oceanography;State Oceanic Administration(Grant No.2014008)

摘　　要：The first successful Agrobacterium-mediated transformation of the green alga Haematococcus pluvialis using the vectors hosting the genes coding for VHB （ Vitreoscilla hemoglobin） and GLUT1 （glucose transporter 1 ） is reported here. The greenish yellow fluorescence of EGFP was observed when the zeocin-resistant cells were viewed with a fluorescent microscope. The functional expression of EGFP showed that the Agrobacterium-mediated transformation could efficiently transfer the exogenous gene into H. pluvialis. RT-PCR was used to successfully amplify the mRNA of VHB and GLUT1 genes from transformed cells, while Southern blots indicated the integration of VHB and GLUT1 genes into the genome of H. pluvialis. Transferring VHB and GLUT1 genes into this alga would pave the way for manip- ulation of many important pathways relevant to the food, pharmaceutical, and nutraceutical industries.The first successful Agrobacterium-mediated transformation of the green alga Haematococcus pluvialis using the vectors hosting the genes coding for VHB （ Vitreoscilla hemoglobin） and GLUT1 （glucose transporter 1 ） is reported here. The greenish yellow fluorescence of EGFP was observed when the zeocin-resistant cells were viewed with a fluorescent microscope. The functional expression of EGFP showed that the Agrobacterium-mediated transformation could efficiently transfer the exogenous gene into H. pluvialis. RT-PCR was used to successfully amplify the mRNA of VHB and GLUT1 genes from transformed cells, while Southern blots indicated the integration of VHB and GLUT1 genes into the genome of H. pluvialis. Transferring VHB and GLUT1 genes into this alga would pave the way for manip- ulation of many important pathways relevant to the food, pharmaceutical, and nutraceutical industries.

关 键 词：Agrobacterium tumefacier~ Genetic transformation Haematococcus pluvialis Vitreoscilla hemoglobin Glucose transporter 1 

分 类 号：Q943.2[生物学—植物学]