PCR Detection of Pilus-associated Genes and Serotype Identification of Pathogenic Escherichia coli Isolated from Chickens in the Jidong Area  

作　　者：Qiumei SHI Yanying ZHANG Xiumin WANG Baoxin YANG Zhiqiang ZHANG Xiaojiao Hou Xinhua SHAO Xia MENG Yang YANG Guoqiang ZHU 

机构地区：[1]Key Laboratory of Preventive Veterinary Medicine of Hebei Province/Hebei Normal University of Science and Technology,Changli 066600,China [2]Beijing Centre Biology Co.,Ltd.,Beijing 102600,China [3]Hebei Xinhua Keji Pharmaceutical(Group)Corporation,Luancheng 051430,China [4]Yangzhou University,Yangzhou 225009,China

出　　处：《Agricultural Biotechnology》2016年第2期43-45,共3页农业生物技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(31072136);Agricultural Science and Technology Achievement Transformation Project of the Ministry of Science and Technology of China(2012GB2A200044);Project of Shijiazhuang Municipal Science and Technology Bureau(11150093A)

摘　　要：[ Objective] This study aimed to explore the presence of type I pill （fimC gene） and P pill （papC gene） and identify the serotype of pathogenic E. coli isolated from chickens in the Jidong Area. [ Method] Type I pill （fimC gene） and P pill （papC gene） were detected by PCR. The serotype was identified by con- ventional agglutination test. [ Result ] The results showed that 100% of chicken-derived E. coil strains expressed type I pill （fimC gene） ; 39.1% （9/23） of chick- en-derived E. coli strains expressed P pill （papC gene）. In addition, 23 isolates of chicken-derived E. coil were assigned to 14 O serotypes, including O78, O93, O 45, O101, O38, O88, O24, O1, O163, O53, O15, O87, O34 and O29, among which O78 was the dominant serotype that accounted for 42.8% （6/14） of the total strain number. [ Conclusion] Chicken-derived E. coli strains in the Jidong Area belonged to 14 serotypes, and 078 was the dominant serotype; 83.3% of 078 serotvDe E. coli strains expressed both tvDe I Dill and P Dill.[ Objective] This study aimed to explore the presence of type I pill （fimC gene） and P pill （papC gene） and identify the serotype of pathogenic E. coli isolated from chickens in the Jidong Area. [ Method] Type I pill （fimC gene） and P pill （papC gene） were detected by PCR. The serotype was identified by con- ventional agglutination test. [ Result ] The results showed that 100% of chicken-derived E. coil strains expressed type I pill （fimC gene） ; 39.1% （9/23） of chick- en-derived E. coli strains expressed P pill （papC gene）. In addition, 23 isolates of chicken-derived E. coil were assigned to 14 O serotypes, including O78, O93, O 45, O101, O38, O88, O24, O1, O163, O53, O15, O87, O34 and O29, among which O78 was the dominant serotype that accounted for 42.8% （6/14） of the total strain number. [ Conclusion] Chicken-derived E. coli strains in the Jidong Area belonged to 14 serotypes, and 078 was the dominant serotype; 83.3% of 078 serotvDe E. coli strains expressed both tvDe I Dill and P Dill.

关 键 词：CHICKEN Escherichia coli SEROTYPE PILI 

分 类 号：S852.61[农业科学—基础兽医学]