金线吊乌龟多糖的含量测定  

Determination of polysaccharide content from Jinxiandiaowugui

作　　者：尚宁宁[1] 禹亚杰[1] 彭腾[1] 高鹏 

机构地区：[1]成都中医药大学中药材标准化教育部重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137 [2]成都煜泉绿健科技有限公司,四川成都610072

出　　处：《中药与临床》2016年第1期15-16,26,共3页Pharmacy and Clinics of Chinese Materia Medica

摘　　要：目的:测定金线吊乌龟多糖的含量。方法:采用水提醇沉法提取、纯化金线吊乌龟多糖,以葡萄糖标准液绘制标准曲线,用硫酸-苯酚比色法于490 nm波长处测定金线吊乌龟多糖的含量。结果:金线吊乌龟精制多糖得率为2.31%,精制多糖含量为71.78%,金线吊乌龟原药材中总多糖含量为1.66%。结论:利用硫酸-苯酚比色法测定金线吊乌龟多糖含量,简便易行,稳定性、精密度和重复性良好,结果准确。Objective： To detect the content of polysacch.afide from Jinxiandiaowugui. Method： The polysaccharide from Jinxiandiaowugui was extracted and purified by water-extraction and alcohol-precipitation method. The standard curve was established using glucose standard solution. Sulfuric acid-phenol colorimetric method was applied to detect polysaccharide content from Jinxiandiaowugui at the wave length of 490nm. Result： The yield rate of refined polysaccharide from Jinxiandiaowugui was 2.31%. The content of refined polysaccharide was 71.78%. The content of total polysaccharide from original medicinal materials of Jinxiandiaowugui was 1.66%. Conclusion： The sulfuric acid-phenol eolorimetric method used in detection of polysaecharide content from Jinxiandiaowugui is simple and rapid. The result is accurate with high stability, precision and repeatability.

关 键 词：金线吊乌龟 多糖 含量测定 

分 类 号：R284.1[医药卫生—中药学]