HPLC-DAD同时测定白花蛇舌草中齐墩果酸和熊果酸的含量  

Simultaneous determination of oleanolic acid and ursolic acid in Baihuasheshecao by HPLC-DAD

作　　者：王欢[1] 吴莹[1] 黄嫣[2] 李希[1,2] 冯建安[2] 王佳[1] 

机构地区：[1]成都中医药大学药学院,四川成都610075 [2]四川省中医药科学院中医研究所,四川成都610031

出　　处：《中药与临床》2016年第1期20-22,36,共4页Pharmacy and Clinics of Chinese Materia Medica

基　　金：四川省省级公益性科研院所基本科研项目(14-4-422)

摘　　要：目的：建立HPLC-DAD同时测定白花蛇舌草中齐墩果酸和熊果酸含量的方法。方法：采用Spursil C18色谱柱（4.6×250 mm,5μm）,流动相为甲醇-水-冰醋酸-三乙胺（90∶10∶0.03∶0.06）,流速0.5 m L﹒min^-1,柱温35℃,检测波长210 nm,进样量10μL。结果：齐墩果酸和熊果酸分别在0.0218-0.218μg﹒μL^-1（r=0.9997）和0.07312-0.7312μg﹒μL^-1（r=0.9996）范围内线性关系良好,平均回收率分别98.17%（RSD=1.89%）、99.31%（RSD=2.62%）。结论：HPLC-DAD能同时测定白花蛇舌草中齐墩果酸和熊果酸含量,并且该检测方法简便准确、稳定可靠,可用于白花蛇舌草药材的质量控制。Objective： To establish a HPLC-DAD method for simultaneous determination of oleanolic acid and ursolic acid in Baihuasheshecao. Method： The separation was performed on the Spursil C18 column （4.6 mm×250 mm, 5 μm） with methanol- water-glacial acetic acid-triethylamine （90：10：0.03：0.06） as mobile phase. The detection wavelength was 210 nm. The flow rate was 0.5 mL· min^-1. The column temperature was 35 ℃, and the injection volume was 10 μL. Result： The linear response of oleanolic acid and ursolic acid were 0.0218-0.218μg·μL^-1 （r=0.9997） and 0.07312-0.7312 μg· μL^-1 （r=0.9996） respectively. The average recovery of oleanolic acid and ursolic acid were 98.17% and 99.31%, with RSD of 1.89% and 2.62% respectively. Conclusion： HPLC-DAD can be used to simultaneously detect oleanolic acid and ursolic acid in Baihuasheshecao. The detection method is simple, accurate, reliable and can be used for quality control of Baihuasheshecao.

关 键 词：白花蛇舌草 HPLC-DAD 齐墩果酸 熊果酸 

分 类 号：R284.1[医药卫生—中药学]