HPLC测定连栀矾溶液中表小檗碱、黄连碱、巴马汀和盐酸小檗碱的含量  

Quantitative analysis of epiberberine, coptisine, palmatine and berberine in Lianzhifan Solution by HPLC

作　　者：袁学刚[1] 王战国[2] 胡慧玲[3] 杨向东[1] 邹亮[2] 余腾江[1,4] 刘巧[3] 

机构地区：[1]成都肛肠专科医院,四川成都610037 [2]成都大学医学院(护理学院),四川成都610106 [3]成都中医药大学药学院,四川成都611137 [4]泸州医学院附属中医院,四川泸州646000

出　　处：《中药与临床》2016年第1期31-33,共3页Pharmacy and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金青年基金项目(81403178);四川省科技厅项目(2015SZ0039;2015FZ0045;2013JY0194);成都市卫生局项目(20140301)

摘　　要：目的：建立快速测定连栀矾溶液中表小檗碱、黄连碱、巴马汀和盐酸小檗碱含量的HPLC方法。方法：色谱柱为Chromstar TM C18色谱柱（4.6 mm×250 mm,5μm）;流动相为乙腈-含0.1%三乙胺和0.1%甲酸的水溶液（30：70,V/V）;检测波长345 nm,柱温30℃,流速0.8 m L﹒min-1。进样量20μL。结果：连栀矾溶液样品中表小檗碱、黄连碱、巴马汀和盐酸小檗碱在12 min内达到分离,峰形尖锐对称。盐酸小檗碱在2.82-44.91μg﹒m L-1范围内线性关系良好,平均加样回收率为97.95%。七批次连栀矾溶液中表小檗碱、黄连碱、巴马汀和盐酸小檗碱含量以盐酸小檗碱计,分别为0.126±0.019,0.167±0.029,0.140±0.018和0.618±0.079 mg﹒m L-1。结论：建立的HPLC方法高效、准确,经济,精密度好,灵敏度高,可用于连栀矾溶液中表小檗碱、黄连碱、巴马汀和盐酸小檗碱的同时快速测定分析。Objective： To establish a HPLC method for quantitative analysis of epiberberine, coptisine, palmatine and berberine in Lianzhifan solution. Method： HPLC separation was performed on a Chromstar TM C18 column （4. 6 mm × 250 mm, 5 μn） with 0.8 mL·mL^-1 mobile phase, which consisted of acetonitrile-water （0.1% triethylamine and 0.1% formic acid, v/v= 30：70）. And the detection was set at 345 nm. Result： Epiberberine, coptisine, palmatine and berberine in Lianzhifan solution were separated well in 12 min. The linearity range of berberine was 2.82-44.91 μg·mL^-1 with high linear regression constant. The average recovery was 97.95%. The contents of epiberberine, coptisine, palmatine and berberine of 7 batches Lianzhifan solution were 0.126 ± 0.019, 0.167 ± 0.029, 0.140 ±0,018, 0.618± 0.079 mg·mL^-1. Conclusion： The established HPLC method was efficient, accurate, economical, good precision and high sensitivity. It can be used for rapid quantitative analysis of epiberberine, coptisine, palmatine and berberine in Lianzhifan solution.

关 键 词：高效液相色谱 连栀矾溶液 表小檗碱 黄连碱 巴马汀 盐酸小檗碱 

分 类 号：R283.6[医药卫生—中药学]