同步半硝化-厌氧氨氧化-反硝化一体式反应器的运行条件和微生物丰度研究  

STUDY ON OPERATION CONDITIONS AND MICROBIAL ABUNDANCE IN SIMULTANEOUS PARTIAL NITRITATION-ANAMMOX-DENITRIFICATION SYSTEM

作　　者：范强[1] 刘国华[1] 徐相龙 罗雅谦 齐鲁[1] 王洪臣[1] 

机构地区：[1]中国人民大学环境学院,北京100872

出　　处：《环境工程》2016年第5期6-10,40,共6页Environmental Engineering

基　　金：中国人民大学科学研究基金(中央高校基本科研业务费专项资金资助)项目(13XNLF04)

摘　　要：通过小试考察了同步半硝化-厌氧氨氧化-反硝化(SNAD)系统的运行条件以及各种细菌的丰度变化情况。结果表明:通过控制温度等运行参数可以成功启动SNAD系统。在启动阶段,细菌的丰度基本保持不变;在稳定化运行阶段,氨氧化细菌(AOB)的丰度为(2.95E+07)copies/g(每克污泥,下同)、亚硝酸盐氧化细菌(NOB)中的Nitrospira和Nitrobacter的丰度分别为(5.87E+05),(3.95E+06)copies/g,厌氧氨氧化(Anammox)细菌的丰度达到了(7.85E+09)copies/g。对于整个系统而言,AOB和Anammox是系统中的优势细菌。The operation conditions and bacterial abundance in a simultaneous partial nitritation-anammox-denitrification（ SNAD） system were evaluated. Results showed that the SNAD system could be started up and operated steadily through controlling temperature,dissolved oxygen（ DO）,aeration mode and biomass. From the half-nitrification to anammox start-up phase,the bacterial abundance in the system remained unchanged. In the stable operation phase,the abundance for ammoniaoxidizing bacteria（ AOB） 、Nitrospira,Nitrobacter and Anammox bacteria were（ 2. 95 E ＋ 07）,（ 5. 87 E ＋ 05）,（ 3. 95 E ＋06） and（ 7. 85 E ＋ 09） gene copies / g-sludge,respectively. Thus,the AOB and Anammox were dominant bacteria,which ensured that the SNAD system could be operated steadily.

关 键 词：一体化厌氧氨氧化 半硝化 条件控制 丰度 qPCR 

分 类 号：X703[环境科学与工程—环境工程]