检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:孙大康[1] 宋向芹[2] 安新业[2] 程艳丽[3]
机构地区:[1]滨州医学院附属医院临床医学实验室,滨州256603 [2]滨州医学院附属医院检验科,滨州256603 [3]滨州医学院附属医院心血管内科,滨州256603
出 处:《现代免疫学》2016年第3期213-218,共6页Current Immunology
基 金:山东省自然科学基金项目(ZR2012CM009);山东省科技发展计划项目(2011YD18015);山东省高等学校科技计划(J11LF89);滨州医学院附属医院青年科研基金(2013QNKYJJ02)
摘 要:比较p24-DsRed1、p24-DsRed2、p24-DsRed-Monomer三种荧光蛋白的表达强弱,观察p24-DsRed1与TRIM22-EGFP结合物在细胞中的分布特点。构建pDsRed1-N1-p24及pDsRed2-N1-p24真核表达载体,转染HEK293T细胞,经荧光显微镜比较三种p24-DsRed荧光偶联蛋白的表达强度。选择荧光强度最强的pDsRed1-N1-p24与pEGFP-N3-TRIM22共转染HEK293T细胞,荧光显微镜观察两者结合物在细胞中的分布特点。结果显示,经荧光显微镜检测,转染48h后红色荧光蛋白强度由大到小依次为p24-DsRed1、p24-DsRed2、p24-DsRedm。在HEK293T细胞中共转染p24-DsRed1和TRIM22-EGFP,发现两者存在共定位关系,有意思的是两者的结合体能以出胞形式脱离细胞。结果表明,p24-DsRed1荧光蛋白强度显著高于p24-DsRed2和p24-DsRedm,TRIM22-p24结合物能以出芽形式脱离细胞。This article aimed to compare the fluorescence intensity of three kinds of HIV capsid p24-DsRed fusion protein and to observe the budding of conjugates of TRIM22 with p24. Plamids pDsRed1-N1-p24 and pDsRed2-N1-p24 were constructed and transfected into HEK293T cells respectively to compare the fluorescence intensity with pDsRedl-N1-Monomer-p24 by a fluo- rescence microscope. Subsequently, plamids pEGFP-N3-TRIM22 and pDsRedl-N1-p24 were co-transfected into HEK293T cells to find out whether TRIM22 could colocalize with p24. Forty-eight hours after transfection, it was showed that the inten- sity of red fluorescence fusion protein,in descending order, was p24-DsRedl, p24-DsRed2, p24-DsRedm. When pEGFP-N3- TRIM22 and pDsRed1-N1-p24 were cotransfected, fluorescence microscopy revealed that TRIM22-EGFP was colicalized with p24-DsRed1 in HEK293T cells. Interestingly, the conjugates of TRIM22 with p24 were released by budding. In conclusion, the fluorescence intensity of p24-pDsRedl was significantly higher than the other two p24-pDsRed proteins and the conjugates of TRIM22-p24 could be released by budding.
关 键 词:HIV衣壳蛋白p24 三基序蛋白22 DSRED 出芽
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:3.145.184.109