SLE患者外周血中CD4^+CXCR5^+T细胞表达ICOS和PD1的相关性研究  

Study on the expression of ICOS and PD1 on CD4^+CRCX5^+T cells in the peripheral blood of patients with systemic lupus erythematosus

作　　者：邢倩[1] 曲世平[1] 金海燕[1] 沈育娟[1] 刘维琳[1] 王革[1] 

机构地区：[1]山东省青岛市市立医院免疫风湿科,青岛266071

出　　处：《现代免疫学》2016年第3期219-223,共5页Current Immunology

基　　金：青岛市科技局项目(13-1-3-19-nsh);青岛市卫生局项目(2012-WSZD003)

摘　　要：本研究检测ICOS、PD-1在SLE患者外周血CD4+CXCR5+T细胞上的表达水平并分析其与SLE临床特征的相关性。采用流式细胞仪检测60例SLE患者及40例健康对照组外周血中CD4+CXCR5+T细胞占CD4+T细胞的比例,同时检测CD4+CXCR5+T细胞上ICOS、PD-1的表达水平,并分析其与SLE疾病活动指数(SLEDAI)、SLE肾脏损害及临床指标之间的相关性。结果显示,SLE患者外周血中CD4+ICOShighT细胞、CD4+ICOShighCXCR5+T细胞、CD4+PD1highCXCR5+T细胞比例显著高于健康对照组(P<0.05);CD4+ICOShighCXCR5+T细胞比例与SLE患者的抗dsDNA抗体水平呈正相关(P<0.05),与SLEDAI评分、免疫球蛋白、C3、C4、ESR、CRP无相关性(P均>0.05);SLE患者血清IL-21水平较健康对照组显著升高(P<0.05)。提示,循环外周血中生物标志分子高表达的CD4+CXCR5+T细胞异常,可能参与SLE的发病。We aimed to investigate the expression of inducible T cell costimulator （ICOS）and programmed death 1 （PD-1） on CD4^＋ CXCR5^＋ T cells from patients with systemic lupus erythematosus （SLE） and analyze their relevance to disease severity. Frequency of CD4^＋ CXCR5^＋ T cell were analyzed by flow cytometry in 60 SLE patients and 40 healthy controls. The expression of ICOS and PD1 on CD4^＋ CXCR5^＋ T cells was analyzed. The results showed that levels of circulating CD4^＋ CXCR5^＋ T cells with high expression of ICOS or PD-1 were increased in patients with SLE compared with that in the healthy controls （P〈 0.05）. The cellular phenotype did not vary with disease activity, serum ]gG concentration , the levels of C3 or C4, ESR, or CRP, but it did correlate with the titers of anti-dsDNA antibodies. The level of IL-21 was increased in patients with SLE com- pared with that of the healthy controls（P^0.05）. These results demonstrate that the abnormality of CD4^＋ CXCR5^＋ T cells with high expression of ICOS or PD-1 may play an important role in the pathogenesis of SLE.

关 键 词：系统性红斑狼疮 CXCR5 ICOS PD1 

分 类 号：R593.24[医药卫生—内科学]