S_(3307)和DTA-6对大豆叶片生理活性及产量的影响  

Effects of plant growth regulators S_(3307) and DTA-6 on the leaf physiological activity and yield of soybean

作　　者：刘春娟[1] 冯乃杰[1] 郑殿峰[1] 孙福东[1] 刘涛[1] 崔洪秋[1] 赵晶晶[1] 

机构地区：[1]黑龙江八一农垦大学农学院,黑龙江大庆163319

出　　处：《植物营养与肥料学报》2016年第3期626-633,共8页Journal of Plant Nutrition and Fertilizers

基　　金：国家自然科学基金项目(31171503;31271652);国家科技支撑计划项目(2012BAD20B04);黑龙江省杰出青年基金项目(JC201309);黑龙江农垦总局科技攻关项目(HNK12A-06-03;HNK12A-09-02);黑龙江省研究生创新科研项目(YJSCX2012-253HLJ);黑龙江八一农垦大学研究生创新科研项目(YJSCX2014-Y04)资助

摘　　要：[目的]化控技术是提高作物产量的一项重要技术,研究植物生长调节剂对大豆鼓粒期至成熟期叶片生理活性及产量的影响,旨在探讨调节剂提高大豆产量的作用机理,为调节剂在农业生产上的应用提供科学依据。[方法]于2013-2014年在黑龙江八一农垦大学林甸试验基地,进行叶面喷施烯效唑（S3307）和2-N,N-二乙氨基乙醇酯（DTA-6）田间试验。试验于初花期（R1期）叶面喷施1次,设3个处理：1）CK,喷施清水;2）喷施DTA-6浓度60 mg/L;3）喷施S3307,浓度50 mg/L。大豆盛荚期（R4期）,用叶绿素仪测定大豆倒3叶和倒9叶的叶绿素值;大豆鼓粒始期（R5期）开始第一次取样,以后每隔5d取样一次,共取样7次,测定叶片中的可溶性糖、蔗糖、淀粉和淀粉酶总活性。大豆成熟期测定产量。[结果]在喷植物生长调节剂后22 d,调节剂极显著增加了大豆植株不同部位,尤其是下部叶片的叶绿素含量,S3307和DTA-6处理的倒3叶叶绿素含量比CK增加9.32%、7.02%,倒9叶叶绿素含量比CK增加11.12%、10.38%,各处理倒3叶和倒9叶的叶绿素含量与产量及产量构成因素均达到正相关。DTA-6和S3307在喷植物生长调节剂后40、45和50 d叶片中的可溶性糖含量均高于CK;DTA-6和S3307处理的蔗糖含量分别在喷植物生长调节剂后4050 d和3550 d达到了最大增加量,S3307对蔗糖积累的作用效果较好;DTA-6和S3307处理在喷植物生长调节剂后50、55和60 d叶片中的淀粉含量均高于CK;不同调节剂处理对淀粉酶总活性也产生了一定影响,喷植物生长调节剂后第45 d时,调节剂作用的淀粉酶总活性达到最大值,各处理淀粉酶活性的高低顺序为S3307、DTA-6〉CK均与CK处理差异显著。不同调节剂处理均增加了单株荚数、粒数、粒重,S3307处理的单株荚数和粒数与CK相比,差异均达到了显著水平,DTA-6处理的各产量因素与CK相比,差异均未达到显著水平。2013年S3307和DTA-6处理【Objectives】Chemicals have played important roles to improve crop yields,proper application will be effective in regulating the physiological metabolism of crops. The study on the effects of plant growth regulators on the leaf physiological activities of soybean will provide scientific basis for their efficient use in soybean production.【Methods】Foliar spray field experiments were conducted successively in 2013 and 2014 in the main soybean producing zone,Heilongjiang Bayi Agricultural University. Experiment was in the early flowering by foliar sprayone time. Two chemical regulators,2- N,N- diethylamino ethyl caproate（ DTA-6,60 mg / L） and uniconazole（ S3307,50 mg / L） were tested with water as control. The first sampling was carried out after sprayed 30 d,and then leaf samples were collected once every five days. The contents of soluble sugar,sucrose,starch and total amylase activity were measured. The grain yields of soybean were investigated. 【Results】Foliar spray of S3307and DTA- 6could significantly increase the chlorophyll content of leaves,especially the lower located ones. In the 22 nd day after spray,the chlorophyll content in the 3rd leaves from the top was increased by 9. 32% and 7. 02% by foliar spray of S3307 and DTA- 6,respectively,and those in the 9th leaves from the top by 11. 12% and 10. 38%respectively. The chlorophyll contents in the third and the ninth leaves from top were significant and positively correlated with yield. The soluble sugar contents in the two leaf samples were always higher than those in control after 40,45 and 50 days of spraying,The sucrose contents reached the maximum at the 40-50 days and 35-50 days since spraying of DTA-6 and S3307,and more sucrose was accumulated in plants sprayed with S3307 than with DTA-6. Starch contents were all higher than the control after the 50 th,55th and 60 th day since the spraying by DTA-6 and S3307. At the 45 th day of spraying,the amylase activity in control decreased to minimum,but those in treatments reached maximum,and

关 键 词：2-N N-二乙氨基乙基己酸酯 烯效唑 大豆 生理活性 产量 

分 类 号：S565.1[农业科学—作物学]