沉默干扰素诱导蛋白16对人主动脉外膜成纤维细胞凋亡的影响  

Reducing Effect of IFI16 Expression on Human Aortic Adventitial Fibroblasts Apoptosis

作　　者：肖燕[1] 吴强[2] 

机构地区：[1]贵州医科大学内科学教研室,贵州贵阳550004 [2]贵州医科大学附属医院人民医院心内科,贵州贵阳550002

出　　处：《贵阳医学院学报》2016年第5期507-510,共4页Journal of Guiyang Medical College

基　　金：国家自然科学基金资助项目(81260030);贵州省科学技术厅-贵州省人民医院科技联合基金项目[黔科合LH字(2015)7159号]

摘　　要：目的:探讨沉默干扰素诱导蛋白16(IFI16)表达对人主动脉外膜成纤维细胞(HAAFs)凋亡的影响及其部分机制。方法:应用IFI16小干扰RAN(siRNA)、Control siRNA转染HAAFs分别IFI16基因沉默组(IFI16-siRNA组)、阴性对照组(Control-siRNA组),未经干预的HAAFs作为空白组;用流式细胞仪检测凋亡,应用Western blot检测IFI16、细胞外调节蛋白激酶1/2(ERK1/2)、磷酸化ERK1/2(p-ERK1/2)蛋白表达。结果:与Control-siRNA组或空白组相比,IFI16-siRNA组的细胞凋亡率下降,伴随着IFI16蛋白表达减少,p-ERK1/2水平增高,差异有统计学意义(P<0.05)。结论:沉默IFI16表达可减少HAAFs细胞凋亡,其机制可能部分与ERK信号通路有关。Objective： To investigate the effect of silencing interferon-inducible protein 16（ IFI16）expression on the apoptosis of human aortic adventitial fibroblasts（ HAAFs）. Methods： The specific small interference RNAs（ siRNAs） of IFI16 and Control siRNA were transected into HAAFs in IFI16-siRNA group,negative control group and untreated blank group. Cell apoptosis was analyzed by flow cytometry. The protein levels of IFI16,extracellular signal-regulated kinase（ ERK1 /2） and phosphorylated extracellular signal-regulated kinase（ p-ERK1 /2） were measured by Western Blot. Results：The protein expression levels of IFI16 were decreased in the HAAFs,the cell apoptosis was downregulated,the protein expression levels of p-ERK1 /2 were increased,differences were statistic significant（ P〈0. 05）. Conclusion： Inhibition of IFI16 expression can downregulate HAAFs cell apoptosis,which may be related to the ERK signaling pathway.

关 键 词：干扰素诱导蛋白 血管外膜成纤维细胞 凋亡 

分 类 号：R541[医药卫生—心血管疾病]