下调干扰素诱导蛋白16对人主动脉外膜成纤维细胞凋亡的影响  

Downregulating Effect of IFI16 Expression Downregulating on Human Aortic Adventitial Fibroblasts Apoptosis

作　　者：肖燕[1] 吴强[2] 

机构地区：[1]贵州医科大学内科学教研室,贵州贵阳550004 [2]贵州医科大学附属人民医院心内科,贵州贵阳550002

出　　处：《贵阳医学院学报》2016年第5期511-514,共4页Journal of Guiyang Medical College

基　　金：国家自然科学基金资助项目(81260030);贵州省科学技术厅;贵州省人民医院科技联合基金项目[黔科合LH字(2015)7159号]

摘　　要：目的:探讨下调干扰素诱导蛋白16(IFI16)对人主动脉外膜成纤维细胞(HAAFs)凋亡的影响及机制。方法:在HAAFs中转染IFI16小干扰RNA(siRNA)和Control siRNA,分别作为IFI16基因沉默组(Ⅰ组)、阴性对照组(C组),未经任何干预的HAAFs作为空白组(N组),用流式细胞仪检测凋亡,Western blot检测IFI16、活化凋亡蛋白聚腺苷二磷酸核糖聚合酶(Cleaved PARP)蛋白表达。结果:与C组和N组相比,Ⅰ组中IFI16蛋白表达减少,细胞凋亡率下降(P<0.05),伴随着Cleaved PARP蛋白表达减少。结论:下调IFI16表达可抑制HAAFs细胞凋亡,其机制可能与下调Cleaved PARP蛋白有关。Objective： To investigate the effect and mechanism of silencing interferon-inducible protein 16（ IFI16） on the apoptosis of human aortic adventitial fibroblasts（ HAAFs）. Methods： The specific small interference RNA（ siRNA） and Control siRNA of IFI16 were transfected into HAAFs in vitro instantaneously. HAAFs were divided into three groups,IFI16 siRNA transfection group（ Ⅰgroup）,negative control group（ C group）,untreated HAAFs as blank group（ N group）. Then cell apoptosis was analyzed by flow cytometry. The protein levels of IFI16,Cleaved PARP were measured by Western Blot. Results： Comparing with group C and N,the protein expression levels and IFI16 were decreased in group I,the cell apoptosis was downregulated（ P〈0. 05）,accompanied with decrease of protein expression of Cleaved PARP. Conclusion： Downregulating of IFI16 expression can inhibit HAAFs cell apoptosis,which may be partially related to the downregulating of Cleaved PARP expression.

关 键 词：干扰素诱导蛋白 成纤维细胞 细胞 主动脉 RNA 小干扰 凋亡 

分 类 号：R541[医药卫生—心血管疾病]