1-磷酸鞘氨醇对雄性小鼠生殖毒性损害的拮抗作用  

Study on Antagonistic Effect of S1P on Toxicity Damage to Male Mice' Reproductive Cells by Cyclophosphamide

作　　者：刘丹薇[1] 潘卫[1] 徐国宾[2] 杨国珍[1] 

机构地区：[1]贵州医科大学医学检验学院生化学教研室,贵州贵阳550004 [2]北京大学肿瘤医院检验科,北京100142

出　　处：《贵阳医学院学报》2016年第5期515-519,共5页Journal of Guiyang Medical College

基　　金：国家自然科学基金(81560720)

摘　　要：目的:探讨1-磷酸鞘氨醇(S1P)对雄性小鼠生殖细胞毒性损害的拮抗作用。方法:健康雄性昆明小鼠40只,随机分为正常对照组、S1P低剂量(0.05μg/g)、S1P中剂量(0.1μg/g)、S1P高剂量(0.2μg/g)组以及环磷酰胺染毒组;正常对照组予生理盐水,后4组予环磷酰胺,腹腔注射给药5 d;S1P低、中、高剂量组后再予相应剂量S1P腹腔注射,给药结束后第30天处死小鼠,分别采集血清、附睾及睾丸样本,检测小鼠睾丸精子计数和精子畸形率,比色法测定LDH活力,彗星实验检测精子DNA损伤。结果:与环磷酰胺染毒组相比,各S1P剂量组精子计数升高,畸形率降低,LDH活力升高,DNA损伤减低,差异有统计学意义(P<0.05)。结论:S1P对雄性小鼠生殖细胞毒性损害具有一定的拮抗作用,能提高精液质量与能量代谢酶LDH的活性,一定程度上抵御细胞DNA损伤。Objective： To study the antagonistic effect of S1 P on toxicity damage to male mouse reproductive cells. Methods： Forty healthy male Kunming mice were randomly divided into 5 groups：normal control group（ physiological saline）,low S1 P dose group（ 0. 05 μg / g）,middle S1 P dose group（ 0. 1 μg / g）,high S1 P dose（ 0. 2 μg / g） and cyclophosphamide exposure control group. Mice in each group were given intraperitoneal injection for 5 days and killed on the 30 th day after the first treatment. The serum,epididymis and testis of the mice were collected. The sperm count and the rate of sperm deformity were detected. The colorimetric method was adopted to detect LDH activity and the comet assay adopted to detect DNA damage of sperm. Results： Compared with cyclophosphamide exposure control group,sperm count increased,deformity rate decreased,LDH activity and cell DNA damage decreased in each experimental S1 P group,and the differences between the two groups were statistically significant（ P〈0. 05）. Conclusion： S1 P has antagonistic effect on toxicity damage to male mouse reproductive cells,can improve semen quality,activate energy metabolism enzyme LDH and resist DNA damage of sperm to some degree.

关 键 词：小鼠 生殖细胞 药物拮抗作用 1-磷酸鞘氨醇 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]