积雪草酸对颅脑损伤的保护作用及其机制研究  

Protective effects and mechanism of Asiatic Acid on traumatic brain injury

作　　者：顾申勇[1,2] 朱秋珍[1] 王静[3] 刘玉兰[1] 张悦[3] 芮耀诚[1] 章越凡[1] 李铁军[1] 

机构地区：[1]第二军医大学药学院药理学教研室,上海200433 [2]复旦大学附属中山医院青浦分院药剂科,上海201700 [3]安徽中医药大学药学院,安徽合肥230031

出　　处：《药学实践杂志》2016年第3期206-209,288,共5页Journal of Pharmaceutical Practice

基　　金：上海市青浦区卫生和计划生育委员会科研课题(W2015-02)

摘　　要：目的研究积雪草酸对颅脑损伤的保护作用及其机制。方法采用落重法建立大鼠闭合性颅脑损伤模型和液压冲击颅脑损伤模型,测定大鼠脑组织含水量、血脑屏障通透性、以及观察脑组织病理形态学变化,研究积雪草酸对颅脑损伤的保护作用;用酶联免疫吸附测定(ELISA)和聚合酶链式反应(PCR)检测炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)的表达,探讨其作用机制。结果积雪草酸可显著减少颅脑损伤后脑组织含水量,减轻血脑屏障通透性,改善脑组织细胞形态;并且可以显著抑制颅脑损伤后炎症因子TNF-α、1L-1β、IL-6的表达。结论积雪草酸具有保护颅脑创伤的作用,其作用机制可能与其抑制炎症作用有关。Objective To study the protective effects and mechanism of Asiatic Acid （AA） on traumatic brain injury.Methods The closed cranioeerebral injury model made by the weight-drop method and fluid percussion brain injury model were used in the study. Brain water content, blood brain barrier permeability and brain tissue pathology morphology were measured to study the neuroprotective effect of AA on traumatic brain injury. Moreover, the inflammatory cytokines such as TNF-α,II.-β,IL-6 were examined by PCR and EI.ISA to study the mechanism of AA. Results Brain water content and permeability of blood brain barrier were significantly decreased, the traumatic brain injury was nameliorated by AA. Moreover, the expressions of inflammatory cytokines TNF-α, IL-β, IL.-6 were inhibited by AA. Conclusion AA has the protective effects on traumatic brain injury and the mechanism is related to the anti inflammatory effect.

关 键 词：积雪草酸 颅脑损伤 炎症 

分 类 号：R285[医药卫生—中药学]