灵芝真菌免疫调节蛋白在体外对不同胃癌细胞的抑制作用  

Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp.on different gastric cancer cell lines in vitro

作　　者：许燕[1,2] 胡明慧 高蓓蓉 周选围[3] 

机构地区：[1]阿斯利康中国创新中心,上海201203 [2]复旦大学生命科学学院,上海200438 [3]上海交通大学农业与生物学院,上海200240

出　　处：《药学实践杂志》2016年第3期223-226,277,共5页Journal of Pharmaceutical Practice

基　　金：国家自然科学基金面上项目(30771500)

摘　　要：目的通过体外试验测定了3种灵芝免疫调节蛋白(FIP-glu、FIP-gat和FIP-SN15)对26株人胃癌细胞抑制增殖的影响。方法采用MTS比色法检测了3种灵芝免疫调节蛋白对26株胃癌细胞的抑制作用,计算出每种灵芝免疫调节蛋白对不同胃癌细胞50%生长抑制所需的药物浓度值(GI_(50))。结果灵芝免疫调节蛋白加入72 h后,发现3种真菌免疫调节蛋白分别对7株胃癌细胞株NUGC-3、GTL-16、HGC-27、IM95m、SNU-638、SNU-216和SNU-5表现出较强的抑制作用,其GI_(50)<50μg/ml。只对2株胃癌细胞NUGC-4和OCUM-1没有抑制作用。结论灵芝免疫调节蛋白在体外对大多数人胃癌细胞株有较强的抑制作用,3种灵芝免疫调节蛋白的作用趋势相同,有进一步开发成抗胃癌药物的潜力。Objective To study the cell proliferative effects of fungal immunomodulatory proteins from Ganoderma spp. on 26 gastric cancer cell lines in vitro. Method 26 human gastric cancer cell lines were treated with FIPs by MTS assay. The average optical density（OD） in 490 nm and inhibition rate（GI50 ）was counted by Universal Microplate Spectrophotometer. Results Three FIPs showed similar profiling in 26 human gastric cancer cell lines after 72 h treatment in cell proliferation as say, which except for NUGC-4 and OCUM 1 did not showed obvious anti proliferative effect, the other 24 human gastric cell lines showed some anti proliferative effects, especially for 7 cell lines（NUGC-3, GTL-16, HGC-27, IM95m, SNU-638, SNU- 216 and SNU-5） showing strong potency, with their GI50 less than 50 μg/ml. Conclusion FIPs showed strong anti prolifera- tive effects in some human gastric cancer cell lines in vitro, which had potential to be further developed as anti gastric cancer drugs.

关 键 词：灵芝 真菌免疫调节蛋白 胃癌细胞 细胞增殖 细胞毒性 MTS比色法 

分 类 号：R965[医药卫生—药理学]