黄连素对IEC-6细胞株增殖与凋亡的影响  

Effect of Berberine on Proliferation and Apoptosis of IEC-6 Cells

作　　者：刘文科[1] 仝小林[1] 冯春鹏[1] 邓岚[1] 李敏[1] 

机构地区：[1]中国中医科学院广安门医院,北京100053

出　　处：《中药新药与临床药理》2016年第3期313-316,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金项目(81102717)

摘　　要：目的观察黄连素对IEC-6细胞增殖和凋亡的影响。方法采用水溶性四氮唑^(-1)(WST^(-1))比色法观察黄连素对IEC-6细胞增殖的影响;采用Annexin V-FITC/PI双标流式细胞术检测黄连素对IEC-6细胞凋亡的影响。结果与正常胎牛血清培养组比较,黄连素低浓度组细胞存活率显著增加,其中1μg·m L^(-1)黄连素组促增殖作用最显著,细胞存活率为220.17%,故选用1μg·m L^(-1)黄连素浓度作为工作浓度。流式细胞术检测结果显示,黄连素干预24 h后,不同葡萄糖状态下细胞的凋亡率均明显下降。与高糖组比较,黄连素高糖组细胞凋亡率下降了6.23%;与低糖组比较,黄连素低糖组细胞凋亡率下降了8.51%;与超高糖组比较,黄连素超高糖组细胞凋亡率下降了9.30%。结论黄连素能够促进肠道L细胞系IEC-6细胞株增殖,并减少其凋亡。Objective To observe the effects of berberine on the proliferation and apoptosis of IEC- 6 cells. Methods The proliferation of IEC- 6 cells was observed by water- soluble tetrazolium^（-1）（WST^（-1）） assay. The apoptosis of IEC- 6cells was observed by flow cytometry through fluorescein annexin V- FITC/PI double labeling. Results The viability of IEC- 6 cells was significantly increased after intervention with berberine as compared with normal culture group.Berberine at 1 μg·m L^（-1）had the strongest effect on promoting the proliferation of IEC- 6 cells,with a survival rate of220.17 %. Therefore, the working concentration of berberine solution was named as 1 μg·m L^（-1）. The flow cytometry results showed that apoptotic rate of IEC- 6 cells at different concentrations of glucose decreased significantly after berberine intervention for 24 h. Compared with high glucose group,the apoptotic rate of high glucose＋berberine group decreased by 6.23 %. Compared with the low glucose group, the apoptotic rate of low glucose＋berberine group decreased by 8.51 %. Compared with the super- high glucose group, the apoptotic rate of super- high glucose＋berberine group decreased by 9.30 %. Conclusion Berberine could promote the proliferation of IEC- 6 cells, and reduce the apoptosis of IEC- 6 cells.

关 键 词：黄连素 IEC-6细胞 增殖 凋亡 

分 类 号：R285.5[医药卫生—中药学]