毛细管气相色谱法同时测定活络油中8种成分的含量  

Determination of Eight Components in Huoluo Oil by Capillary Gas Chromatography

作　　者：张润容 叶雪峰 陈海燕 

机构地区：[1]丽珠医药集团股份有限公司,广东珠海519000 [2]珠海宏利药业有限公司,广东珠海519085

出　　处：《中药新药与临床药理》2016年第3期413-417,共5页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立活络油中有效成分α-蒎烯、桉油精、樟脑、薄荷脑、水杨酸甲酯、桂皮醛、丁香酚、麝香草酚的气相色谱测定法,为该制剂的质量控制提供参考。方法采用气相色谱法,色谱条件以PEG-20M毛细管柱(30 m×0.25 mm×0.25μm),程序升温:起始温度80℃,保持3 min,每分钟升15℃,终止温度180℃,保持10 min,分流比50:1,FID检测器。结果线性范围:α-蒎烯0.50~7.54 mg·mL^(-1),桉油精0.51~7.59 mg·mL^(-1),樟脑0.16~2.44 mg·mL^(-1),薄荷脑0.67~10.01 mg·m L^(-1),水杨酸甲酯1.17~17.54 mg·mL^(-1),桂皮醛0.16~2.47 mg·mL^(-1),丁香酚0.34~5.04 mg·mL^(-1),麝香草酚0.17~2.51 mg·mL^(-1),回归方程的相关系数在0.99994~0.99998之间。结论本研究所采用的气相色谱法操作快速、简便、准确,精密度、重复性、稳定性好,可用于活络油的质量控制。本方法也可适用于含有类似成分的药油的检测和质量评价。Objective To develop a capillary gas chromatography（GC） method for determining active components in Huoluo Oil, α- pinene, eucalyptol, camphor, menthol, methyl salicylate, cinnamic aldehyde, eugenol and thymol, thus to provide a reference for quality control of Huoluo Oil. Methods GC was applied for the quantitative analysis. GC was performed on PEG- 20 M capillary chromatographic column（30 m ×0.25 mm ×0.25 μm） by temperature- programmed manner. The initiation temperature was set at 80℃ for 3 min,the rate of temperature rising was 15℃ /minute, and final temperature was kept at 180 ℃ for 10 min. The bypass rate was 50：1, and flame ionization detector（FID） was adopted. Results The standard curve was linear for α- pinene within the ranges of0.50~7.54 mg·mL^（-1）,0.51~7.59 mg·mL^（-1） for eucalyptol,0.16~2.44 mg·mL^（-1） for camphor,0.67~10.01 mg·m L^（-1） for menthol,1.17~17.54 mg·m L^（-1） for methyl salicylate,0.16~2.47 mg·m L^（-1） for cinnamic aldehyde,0.34~5.04 mg·m L^（-1）for eugenol and 0.17~2.51 mg·mL^（-1） for thymol. The correlation coefficients of regression equation were between0.99994~0.99998. Conclusion The established GC is rapid, simple and accurate, and with good precision,repeatability and stability,which is suitable for the quality control of Huoluo Oil. The method can also be applied to control the quality of other herbal medicinal oil products.

关 键 词：毛细管气相色谱法 活络油 Α-蒎烯 桉油精 樟脑 薄荷脑 水杨酸甲酯 桂皮醛 丁香酚 麝香草酚 

分 类 号：R284.1[医药卫生—中药学]