吉林地区人群HPA-1～6,15系统基因多态性分析  

HPA-1～6,15 system gene polymorphism analysis in jilin

作　　者：杨帆[1] 韩瑜[1] 焦立新[1] 鲁威[1] 金成日[1] 陈琳[1] 牛潼 李俊玮[2] 

机构地区：[1]吉林省血液中心,吉林长春130033 [2]吉林大学临床医学院

出　　处：《中国实验诊断学》2016年第5期750-751,共2页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省卫生青年科研课题(2013Q016);吉林省自然科学基金项目(201015186);吉林省卫生自筹经费课题(2013ZC010)

摘　　要：目的调查吉林地区人群人类血小板抗原（HPA）基因多态性。方法采用PCR-SSP方法对419名血小板捐献志愿者进行血小板抗原HPA-1-6,15系统基因分型,通过统计方法对分型结果进行基因多态性分析。结果血小板抗原HPA-1-6,15系统基因频率分别为HPA-1aa 0.984,HPA-1ab 0.016,HPA-2aa 0.872,HPA-2ab 0.123,HPA-2bb 0.004,HPA-3aa 0.350,HPA-3ab 0.483,HPA-3bb 0.166,HPA-4aa 0.992,HPA-4ab 0.008,HPA-5aa0.996,HPA-5ab 0.004,HPA-6aa 0.984,HPA-6ab 0.016,HPA-15aa 0.318,HPA-15ab 0.492,HPA-15bb 0.190。结论吉林地区人群HPA-1-6和HPA-15系统基因多态性具有本地区特点,HPA-3和HPA-15系统不配合率最高,HPA-2系统次之。Objective Survey the crowd in jilin area human platelet antigen（HPA）gene polymorphism.MethodsThe PCR-SSP method in 419 platelet donors for genotyping antigen HPA_1-6,15 system,the statistical method to classification results for gene polymorphism analysis.Results The frequency of HPA pherotype is HPA-1aa 0.984,HPA-1ab 0.016,HPA-2aa 0.872,HPA-2ab 0.123,HPA-2bb 0.004,HPA-3aa 0.350,HPA-3ab 0.483,HPA-3bb 0.166,HPA-4aa 0.992,HPA-4ab 0.008,HPA-5aa 0.996,HPA-5ab 0.004,HPA-6aa 0.984,HPA-6ab 0.016,HPA-15aa0.318,HPA-15 ab 0.492,HPA-15 bb 0.190.Conclusion HPA-1-6,15 system are polymorphic gene frequency distribution,the HPA-3and 15 system,high heterozygosity,it is worth noting that the HPA-2gene.

关 键 词：人类血小板抗原 基因型 PCR-SSP 

分 类 号：R446.1[医药卫生—诊断学] R440[医药卫生—临床医学]