血清半乳糖凝集素3在2型糖尿病并发冠状动脉疾病患者中的诊断价值  

Diagnostic value of plasma galectin-3 in patients with type 2 diabetes mellitus complicated with coronary artery disease

作　　者：周芳[1,2] 王金祥[1] 王珂[3] 朱向情[1] 庞荣清[1] 李雪梅[3] 朱光旭[1] 潘兴华[1] 

机构地区：[1]成都军区昆明总医院细胞生物治疗中心细胞生物医药技术国家地方联合工程实验室云南省干细胞工程实验室,云南昆明650032 [2]昆明医科大学成都军区昆明总医院临床学院,云南昆明650500 [3]成都军区昆明总医院检验科,云南昆明650032

出　　处：《中国实验诊断学》2016年第4期573-576,共4页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金项目(81170316);国家自然科学基金项目(81101158)

摘　　要：目的探讨2型糖尿病(type 2diabetes mellitus,T2DM)并发冠状动脉疾病(coronary artery disease,CAD)患者血清中半乳糖凝集素(Galectin-3)的表达情况、与超敏C反应蛋白(hypersensitive C reactive protein,hs-CRP)的相关性及其诊断价值。方法采用病例对照研究,选择成都军区昆明总医院CAD患者(CAD组)100例、T2DM并发CAD患者(T2DM-CAD组)100例、T2DM患者(T2DM组)100例及健康对照者(Control组)100例,采集临床标本,分别应用酶联免疫法、免疫比浊法测定Galectin-3和hs-CRP的含量,同时采用酶法检测血糖(Glu)、尿素(BUN)、肌酐(CREA)、尿酸(UA)、甘油三酯(TG)、总胆固醇(TC)的浓度,低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)则运用直接法检测。统计学分析采用K-S检验,Levene方差齐性检验,Kruskal-Wallis方差分析,Nemenyi检验,χ2检验,Pearson相关分析及ROC曲线。结果 T2DM-CAD组Galectin-3和hs-CRP的水平分别为(10.523±4.552)mg/L和(9.60±3.75)mg/L,均明显高于CAD组的(7.417±1.797)mg/L、(5.57±2.21)mg/L和T2DM组的(6.515±1.499)mg/L、(5.23±2.51)mg/L,差异有统计学意义(P均<0.01);T2DM-CAD组的Galectin-3与hs-CRP呈正相关(r=0.724,P<0.001);在T2DM-CAD组/T2DM组,ROC曲线下面积Galectin-3检测为0.859,hs-CRP检测为0.842,差异无统计学意义(Z=0.245,P>0.05)。结论 Galectin-3对于T2DM是否并发CAD具有与hs-CRP相似效能的诊断价值,为临床诊断提供一种新的生物学标记。Objective To investigate the plasma level of Galectin-3,the correlationship between the plasma level of Galectin-3and hypersensitive C reactive protein（hs-CRP）,and to explore the diagnostic value of Galectin-3in patients with type 2diabetes mellitus complicated with coronary artery disease.Methods Case control study was used.The study enrolled 100 patients with coronary artery disease（CAD group）,100 patients with type 2diabetes mellitus complicated with coronary artery disease（T2DM-CAD group）,100 patients with type 2diabetes mellitus（T2DM group）,and 100 healthy subjects with similar age and gender（control group）.The clinical data were collected.Galectin-3and hs-CRP plasma levels were detected by enzyme linked immunosorbent assay and immunonephelometry method respectively.Simultaneously,the plasma levels of glucose（Glu）,blood urea nitrogen（BUN）,creatinine（CREA）,uric acid（UA）,triglyeride（TG）and total cholesterol（TC）were also detected by enzymic method;the plasma levels of low density lipoprotein cholesterol（LDL-C）and high density lipoprotein cholesterol（HDL-C）were detected by direct method.K-S test was used to judge normality of measurement data,Levenetest was used to judge homogeneity of variance of more than three group measurement data,Kruskal-Wallis was used to analysis of variance was used to compare more than three group measurement data,Nemenyi test was used to compare two of several group measurement data,andχ2 test was used to compare several group count data.Pearsontest was used for correlationship analysis between groups.Receiver operator characteristic curve（ROC curve）was used to evaluate diagnostic value of markers.ResultsCompared with the levels of CAD group[Galectin-3：（7.417±1.797）mg/L;hs-CRP：（5.57±2.21）mg/L]and T2 DM group[Galectin-3：（6.515±1.499）mg/L;hs-CRP：（5.23±2.51）mg/L],the plasma levels of Galectin-3and hs-CRP in T2DM-CAD group[（10.523±4.552）mg/L and（9.60±3.75）mg/L respectively]were significantly inc

关 键 词：2型糖尿病并发冠状动脉疾病 半乳糖凝集素3 超敏C反应蛋白 

分 类 号：R587.1[医药卫生—内分泌]