系统性红斑狼疮患者血清维生素D水平与维生素D抗体及自身抗体关系的研究  

Study of Relationships between 25-Hydroxyvitamin D3 and both Vitamin D Antibodies and Autoantibodies in Systemic Lupus Erythematosus

作　　者：邢桂芝[1] 王志敏[2] 石哲群 佟胜全 房成[1] 褚金龙[1] 

机构地区：[1]唐山市工人医院检验科,河北唐山063000 [2]河北大学附属医院风湿免疫科 [3]唐山市康复医疗中心

出　　处：《中国实验诊断学》2016年第4期592-595,共4页Chinese Journal of Laboratory Diagnosis

基　　金：河北省卫生厅科研项目(20150956)

摘　　要：目的观察系统性红斑狼疮(SLE)患者血清25羟基维生素D3(25-OH-D3)水平与疾病活动度的相关性,研究其与维生素D抗体(VD-Ab)及自身抗体的关系,并探讨其临床意义。方法收集资料完整的90例SLE患者及60例健康志愿者的血清标本。应用化学发光法测定血清25-OH-D3水平,酶联免疫(ELISA)法测定血清VD-Ab水平,免疫斑点法测定自身抗体。采用SPSS 13.0软件进行数据分析。结果病例组血清25-OH-D3水平为10.29±5.74ng/ml,对照组血清25-OH-D3水平为13.72±5.39ng/ml,两组比较,差异有统计学意义,P<0.05。病例组血清VD-Ab水平为792.2±450.7nmol/L,对照组为705.3±280.2nmol/L,差异无统计学意义,P>0.05。SLE患者血清25-OH-D3水平与VD-Ab水平无明显相关性(r=-0.120,P>0.05),与SLEDAI呈负相关(r=-0.417,P<0.01)。SLE患者抗dsDNA抗体、抗组蛋白抗体、抗心磷脂抗体阳性组血清25-OH-D3水平低于阴性组,P<0.05。抗nRNP抗体、抗Sm抗体、抗SSA抗体、抗Ro52抗体、抗SSB抗体、抗核小体抗体、抗核糖体P蛋白抗体阳性组与阴性组血清25-OH-D3水平比较差异无统计学意义,P>0.05。结论 SLE患者血清25-OH-D3水平低下,与疾病活动度负相关。SLE患者血清维生素D水平降低与VD-Ab无关,可能与抗dsDNA抗体、抗组蛋白抗体、抗心磷脂抗体有关,维生素D在SLE发病中起重要作用。Objective To observe the associations of Serum 25-hydroxyvitamin D3（25-OH-D3）levels with disease activity inPatients with systemic lupus erythematosus（SLE）,study the relationships between 25-OH-D3 and both vitamin D-antibody（VD-Ab）and autoantibodies,and investigate their clinical significance.Methods To collect serum samples of 90 cases of SLE patients whose data complete and 60 cases of healthy volunteers.Applicating chemiluminescence to determinate the level of serum 25-OH-D3,the Enzyme-linked Immunosorbent Assay（ELISA）method was employed to detect the level of serum VD-Ab,applicating immune spot to determinate autoantibodies.Data analysis is done with SPSS 13.0statistical software.Results The average level of serum 25-OH-D3 of patient group was 10.29±5.74ng/ml,and control group was 13.72±5.39ng/ml,compare it between them,showed P0.05,the difference was statistically significant.The serum VD-Ab level in patient group was 792.2±450.7nmol/L,and the level in control group was705.3±280.2nmol/L,it not reach statistical significance,P0.05.There was no significant relationships between the level of serum 25-OH-D3 and the level of serum VD-Ab（r=-0.120,P0.05）,a negative correlation was found between the levels of 25-OH-D3 and SLEDAI（r=-0.417,P0.01）.The level of serum 25-OH-D3 in SLE patients with anti dsDNA,anti-histone,anticardiolipin antibody positive group was lower than that of the negative group,P0.05.There was no significant difference between the level of serum 25-OH-D3 of anti-nRNP antibody,anti-Sm antibody,anti-SSA antibody,anti-Ro52 antibody,anti-SSB antibody,anti nucleosome antibody,anti-Ribp antibody positive group and that of the negative group,P0.05.Conclusion The level of serum vitamin D in SLE patient is low,and it is negatively correlated with disease activity.The low level of serum 25-OH-D3 is not correlated with anti-vitamin D antibody,it may correlated with anti dsDNA antibody,anti-histone antibody and anticardiolipin antibody,vitamin D is important in the pathogenesis of

关 键 词：系统性红斑狼疮 25-羟-维生素D3 维生素D抗体 自身抗体 

分 类 号：R593.241[医药卫生—内科学]