2014年贵州省手足口病流行病学特征和肠道病毒71型基因亚型分析  

Molecular characteristics of enterovirus 71 and epidemiological characteristics of hand,foot and mouth disease in Guizhou Province,2014

作　　者：李法锦[1] 王丹[2] 李世军[1] 蒋维佳[1] 郭军[1] 王定明[3] 唐光鹏[4] 

机构地区：[1]贵州省疾病预防控制中心传染病防治研究所检验科,贵州贵阳550004 [2]贵州省疾病预防控制中心传染病防治研究所肠道科,贵州贵阳550004 [3]贵州省疾病预防控制中心,贵州贵阳550004 [4]贵州省疾病预防控制中心传染病防治研究所,贵州贵阳550004

出　　处：《中华疾病控制杂志》2016年第5期486-490,共5页Chinese Journal of Disease Control & Prevention

基　　金："十二五"国家科技重大专项(2012ZX1004-212);贵州省普通高等学校传染病与生物安全特色重点实验室项目(黔教合KY字[2015]331)

摘　　要：目的 分析贵州省2014年手足口病（hand,foot and mouth disease,HFMD）的流行病监测信息报告管理系统中的HFMD资料进行分析。方法 对2014年贵州省HFMD病例的EV-71分离株的VP1编码区进行逆转录-聚合酶链式反应,测定扩增产物的核苷酸序列,使用MEGA（molecular evolutionary genetics analysis,MEGA）6.0软件分析VP1区基因变异特征及基因亲缘关系。结果 2014年贵州省HFMD发病数为50 880例,发病率为145.28/10万,死亡率为0.06/10万。发病高峰在4-6月,其中4月发病数最高,6月死亡数最高,2014年每月贵州省各市均有HFMD报告病例,其中遵义和贵阳发病率较高,散居儿童发病率最高,发病年龄主要在0-5岁幼童,发病率为93.81%,发病数男女之比为1.59∶1。实验室确诊HFMD共计3 877例,占总病例数比例为7.62%,其EV-71占实验室确诊病例数的26.41%,CA-16占32.42%,其他EV占40.74%。结论 2014年每月贵州省各市均有HFMD流行,发病高峰在4-6月,以5岁以下散居儿童为主,以EV-71和CA-16共循环为主。2014年在贵州省分离到的EV-71均属于C4基因亚型中C4a进化分支。Objective To analyze the epidemiology of hand,foot and mouth disease（ HFMD） in Guizhou province during 2014,and the genetic characterizations of enterovirus 71（ EV-71）. Methods Statistical analysis for HFMD data reported by National Disease Supervision Information Management System were performed by using descriptive epidemiology method; the VP1-region sequence amplification of EV-71 isolated from HFMD patients in Guizhou province during 2014 were also performed by using reverse-transcription-polymerase chain reaction,then nucleotide of the amplicons were sequenced,finally genetic variability of VP1 region sequences of EV-71 and phylogenetic tree were analyzed by using MEGA（ Molecular Evolutionary Genetics Analysis,MEGA） 6. 0 software. Results The cases of HFMD patients were 50880 in Guizhou province during 2014. The incidence of HFMD patients was 145. 28 /100 000,and the mortality rate was0. 06 /100,000. HFMD patients were reported every month in Guizhou province during 2014,the peak incidence occured mainly during April-June,and the highest incidence city area was in Zunyi and Guiyang in April,the highest deaths was in June,the incidence accounted highest among scattered children. The peak incidence occurs in children mainly aged 0-5years,and the incidence occurs in children was 93. 81%. The proportion of male and famale was 1. 59 ∶ 1. There were 3877 cases in total,which were laboratory-confirmed HFMD,were reported in 2014,the proportion in the total number of cases was 7. 62%; Among them,the constituent ratio of pathogens were described as： EV-71 was 26. 41%,CA-16 was32. 42%,and other EV was 40. 74%. All EV-71 strains isolated in Guizhou Province in 2014 belong to C4 a Phylogenetic branch within C4 genogroup. Conclusions HFMD outbreaks occurred every week in Guizhou Province during 2014,and the morbidity is noticeable in different cities and counties. The peak incidence in each year ranges from April to June. Most of the patients were children under 3 years old,and children who live scattered

关 键 词：手足口病 流行病学 基因 

分 类 号：R512.57[医药卫生—内科学] R181[医药卫生—临床医学]