链霉菌BAF-0711产生的抗肿瘤活性成分的分离纯化和结构鉴定  

Isolation,Purification and Structural Identification of Component with Antitumor Activity Produced by Streptomyces strain BAF-0711

作　　者：张欣[1] 杨煌建[2] 周剑[2] 陈宏[3] 方东升[2] 黄楷[2] 郑卫[1,2] 

机构地区：[1]福建医科大学药学院,福州350004 [2]福建省微生物研究所,福州350007 [3]福建省中医药研究院药物研究所,福州350003

出　　处：《天然产物研究与开发》2016年第5期696-698,649,共4页Natural Product Research and Development

摘　　要：探讨分离、纯化和鉴定链霉菌BAF-0711发酵液中的抗肿瘤活性成分的方法。采用大孔吸附柱层析、硅胶柱层析等方法对该菌株的次级代谢产物进行分离纯化;根据理化性质和波谱学方法进行化学结构的鉴定;利用MTT法来检测化合物的抗肿瘤活性。从该菌株发酵液中分离到化合物BAF-0711-A。经波谱方法鉴定与文献中报道的bafilomycin A1同质。以拉帕替尼为对照,BAF-0711-A对乳腺癌细胞MB-45-3d有较强的增殖抑制活性,其IC50值为0.217μmol/L。The purpose of the present study was to isolate, purify and identify components with antitumor activity produced by Streptomyces sp. BAF-0711. The secondary metabolite was isolated and purified by macroporous resin column chromatography, silica gel chromatography, etc. ;the chemical structure was identified by physicochemical properties and spectroscopic methods ; The antitumor activity was detected by MTT assay. As a result, compound BAF-0711 -A was isolated from the fermentation broth of Streptomyees sp. BAF-0711 and was identified as bafilomyein A1 by spectroscopic methods. MTT assay showed that BAF-0711-A had strong growth inhibitory activity compared with lapatinib on MDA- MB-453 cell,with ICs0 value of 0. 217 μmol/mL.

关 键 词：BAFILOMYCIN A1 大环内脂 分离纯化 结构鉴定 抗肿瘤活性 

分 类 号：R979.1[医药卫生—药品]