基于成分敲除/敲入研究广藿香油抗菌主要药效物质  

Analysis of Main Antibacterial Components in Patchouli Oil Based on Constituent Knock-out/Knock-in

作　　者：赵思蕾 熊亮[1,2] 王振强[1,2] 林美斯 杨华蓉[2] 林大胜 傅超美[1] 

机构地区：[1]成都中医药大学药学院中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137 [2]成都华神集团股份有限公司,成都610075

出　　处：《天然产物研究与开发》2016年第5期707-712,共6页Natural Product Research and Development

基　　金：四川省重大科研支撑项目(2012JY114);四川省教育厅自然科学重点项目(15ZA0084);四川省杰出青年基金(2015JQO030)

摘　　要：为研究广藿香油抗菌主要药效物质,遵循成分敲除/敲入研究模式,以百秋李醇与广藿香酮为目标成分,用广藿香油制备百秋李醇与广藿香酮不同含量比例的样品;采用琼脂平板二倍稀释法,分别测定各样品对6种革兰氏阳性菌种及5种革兰氏阴性菌种的最低抑菌浓度(MIC);采用SAS 9.2软件对所得数据进行一元方差分析,并采用SNK法(q检验)联合析因设计进行两两比较分析。实验结果表明广藿香油抗菌效果显著优于敲除目标成分后的阴性样品,但阴性样品仍具有一定的抗菌效果;广藿香油与阳性对照样品(单体百秋李醇∶广藿香酮=2∶1)、百秋李醇单体、广藿香酮单体之间,其整体抗菌效果基本相当;但百秋李醇对革兰氏阳性菌的抑制作用均优于广藿香酮,广藿香酮对革兰氏阴性菌的抑制作用均优于百秋李醇;且两目标成分不同比例配伍的广藿香油对不同菌种的抗菌效果略有差异。由此可见,百秋李醇与广藿香酮为广藿香油的主要抗菌活性物质,百秋李醇、广藿香酮对革兰氏阳性菌、革兰氏阴性菌抑制作用有所差异,且两者比例差异对广藿香油抗菌作用有一定的影响,随着广藿香酮比例的增加,对革兰氏阳性菌抗菌活性有下降趋势,对革兰氏阴性菌的抗菌活性有增加趋势。To analysis the main antibacterial components in patchouli oil, based on the principle of Knock-out/Knock-in, aiming at the two targeted components（patchouli alcohol and patchottlenone） ,different samples containing different eon- tent and proportion of patchouli alcohol and patchoulenone were prepared. Minimal inhibitory concentration（MIC） of the samples against 6 gram-positive bacteria and 5 gram-negative bacteria were detected by standard ager dilution method. SAS 9.2 software was adopted to do univariate ANOVA, and each different two groups of data were compared by SNK method（q-test） combined with factorial design. The results showed that antibacterial effect of original patchouli oil were significantly better than the knocked-out negative sample; the knocked-out negative sample still remained a certain of an- tibacterial effect though the effect was decreased to some degree;the original patchouli oil, the positive sample（ patchouli alcohol： patchoulenone = 2：1 ）, patchouli alcohol and patchoulenone had almost the same effect;the antibacterial effect of patchouli alcohol against gram-positive bacteria was better than patchoulenone, while patchoulenone showed better effect than patchouli alcohol against gram-negative bacteria;patchouli oil with different proportion achieved quit different antibacterial effect. In conclusion, although other unknown components remain a certain degree of antibacterial effect, patchouli alcohol and patchoulenone were the main antibacterial components of patchouli oil; the two targeted compounds performed quite different activity against gram-positive bacteria and gram-negative bacteria; the effect of patchouli oil slightly changed by adjust the proportion between the two targeted compounds：with the proportion of pat- choulenone increasing,the antibacterial effect trend to decrease for gram-positive bacteria and trend to increase for gramnegative bacteria.

关 键 词：广藿香油 敲除/敲入 百秋李醇 广藿香酮 抗菌 

分 类 号：R285[医药卫生—中药学]