王不留行刺桐碱提取工艺的比较研究  

Comparison of Ultrasonic Extraction and Reflux Extraction of Hypaphorine from Vaccaria segetalis

作　　者：刘艳玲[1] 王旭[2] 侯豹[1] 蔡维维[2] 冯磊[2] 邱丽颖[2] 

机构地区：[1]江南大学药学院 [2]江南大学无锡医学院,无锡214122

出　　处：《天然产物研究与开发》2016年第5期728-734,共7页Natural Product Research and Development

基　　金：中央高校基本科研业务费专项资金(JUSRP51412B);江苏省普通高校研究生科研创新计划(SJLX_0530)

摘　　要：本实验采用高效液相色谱(HPLC)法检测王不留行刺桐碱含量为评价指标,分别采用单因素试验法和正交试验法对水浴回流提取和超声提取对王不留行刺桐碱的提取工艺进行比较。结果表明:超声提取王不留行刺桐碱的最佳工艺参数为液固比10∶1 mL/g,60%乙醇在350 W功率、80℃下提取40 min,在该工艺条件下王不留行刺桐碱含量为0.395 mg/g;水浴回流提取王不留行刺桐碱的最佳工艺参数为乙醇浓度75%,提取温度95℃,提取时间2 h,液固比12∶1 mL/g时,王不留行刺桐碱含量为0.345 mg/g。超声提取王不留行刺桐碱的提取效率、稳定性和重复性都优于水浴回流提取法。In this study,the content of hypaphorine from Vaccaria segetalis determined by high performance liquid chromatography（HPLC） was used as an evaluation index, single factor and orthogonal tests were carried out to optimize of water bath reflux extraction and uhrasonie extraction of hypaphorine from V. segetalis separately. The results showed the optimal parameters for the extraction of hypaphorine from V. segetalis by ultrasonic extraction were： liquid-solid ratio of 10：1 ml/g,60% ethanol as extraction solvent,350 W as ultrasonic power,80℃ as extraction temperature and 40 rain as extraction time. Under these conditions, the content of hypaphorine from V. segetalis was 0. 395 mg/g. The optimal water bath reflux extraction conditions were： ethanol concentration of 75%, extraction temperature of 95℃, extraction time of 2 h and liquid-solid ratio of 12：1 mL/g,the content of hypaphorine from V. segetalis was 0.345 mg/g. The efficiency, stability and repeatability of ultrasonic extraction were better than water bath reflux extraction.

关 键 词：王不留行 刺桐碱 水浴回流提取 超声提取 提取工艺 

分 类 号：O629.36[理学—有机化学] R284.2[理学—化学]