大王马先蒿苯丙素类含量测定及有效部位抗疲劳活性研究  

Determination of Phenylpropanoids and Anti-fatigue Activity of Effective Fractions of Pedicularis rex

作　　者：褚洪标[1] 张志华[1] 陈冬[1] 王茜[1] 

机构地区：[1]井冈山大学医学院,吉安343009

出　　处：《天然产物研究与开发》2016年第5期754-760,共7页Natural Product Research and Development

基　　金：国家自然科学基金(31260084);江西省自然科学基金(20142BAB205024)

摘　　要：建立了大王马先蒿中红景天苷、毛蕊花苷、异毛蕊花苷、米团花苷A、紫地黄苷D和角胡麻苷等6种苯丙素类成分的测定方法,并利用大孔吸附树脂制备有效部位,评价有效部位的抗疲劳、耐缺氧活性。采用ZORBAX-C18（4.6 mm×150 mm,5μm）色谱柱,以水（A）-甲醇（B）溶液为流动相,梯度洗脱（0~10 min,63%A;10~10.5 min,63%→58%A;10.5~40 min,58%A）,流速1 m L/min,检测波长0~4 min为275 nm,4~40 min为330nm,柱温35℃,进样量10μL;利用D101大孔吸附树脂梯度洗脱方法,制备苯丙素类成分有效部位,通过小鼠负重游泳、耐缺氧模型,观察不同剂量组小鼠力竭游泳时间及密闭缺氧存活时间。结果表明6种苯丙素类成分进样量分别在2.10~8.40、13.60~54.40、0.93~3.72、0.53~2.12、1.50~6.00、0.37~1.28范围内线性关系良好,平均加样回收率（n=6）均在98%~102%范围内。有效部位制取率为0.62%,6种苯丙素类成分总含量达到65%以上。高、中、低剂量组小鼠的力竭时间和缺氧存活时间明显高于空白对照组（P〈0.01）。本实验分析方法准确、简便、重复性好,可作为大王马先蒿中6种苯丙素类成分的含量测定方法。苯丙素有效部位能延长小鼠游泳时间,并明显延长小鼠在常压缺氧的存活时间,增强小鼠耐缺氧能力,提示大王马先蒿具有促进恢复和消除疲劳及提高运动能力的效用。The aim of this sttldy was to establish an HPLC method for simultaneous determination of salidroside, verbascoside,iso-verbascoside,leucoseptoside A,jionoside D and martynoside in Pedicularis rex, and to evaluate the anti-fatigue and anoxia endurance activities of P. rex extract. The chromatographic separation was carried out on a ZORBX-C18 （4.6 mm× 150 mm,5 μma） column,with mobile phases：H2O（A） -MeOH（B） （0 - 10 min,63% A;10 - 10.5 min, 63%→58% A;10.5-40 rain,58% A） ,at the flow rate of 1.0 mL/min and detection wavelength was set at 275 nm（0 - 4 min） and 330 nm （4 - 40 min）. The column temperature was maintained at 35℃. The injection volume was 10μL. The effective fractions of pheuylpropanoids were prepared by macroporous adsorption resin. The contents of salidroside, verbascoside,iso-verbascoside,leucoseptoside A,jionoside D and martynoside had good linear relationship with in the ranges of 2.10 - 8.40,13.60 - 54.40,0.93 - 3.72,0.53 - 2.12,1.50 - 6.00,0.37 - 1.28, respectively, and the average recoveries of the 6 phenylpropanoids were all in the range of 98% - 102%. The extraction yield of effective fractions was 0.62% with total contents of phenylpropanoids higher than 65%. The exhaustive swimming time and anoxia endurance time of three dosages groups were higher than those of the vehicle（ P 〈 0.01 ）. The HPLC method was reliable, sim- ple and accurate. It can be used for the quality control of P. rex. This experiment suggested that P. rex had the effect of promoting recovery, eliminating fatigue and improving exercise capacity.

关 键 词：大王马先蒿 苯丙素类 高效液相色谱法 有效部位 抗疲劳 耐缺氧 

分 类 号：R917[医药卫生—药物分析学]