湖南黑茶对小鼠辐射损伤的保护作用  

Protective Effects of Hunan Dark Tea Against Radiation Injuries in Mice

作　　者：张广慧[1] 吴红英[1] 周则卫[1] 张晓东[1] 王浩[1] 刘培勋[1] 龙伟[1] 

机构地区：[1]中国医学科学院北京协和医学院放射医学研究所,天津300192

出　　处：《天然产物研究与开发》2016年第5期775-780,共6页Natural Product Research and Development

基　　金：国家自然科学基金青年项目(81202153);教育部博士点新教师基金(20121106120042);天津市应用基础与前沿技术研究计划青年项目(14JCQNJC10900);中国医学科学院放射医学研究所发展基金(1523)

摘　　要：研究湖南黑茶对辐射损伤小鼠的保护作用。本研究采用外周血液学指标、免疫系统指标、体内抗氧化指标超氧化物歧化酶(Superoxide dismutase,SOD)及存活时间考察黑茶对辐射损伤小鼠的保护作用。结果显示,小鼠受到137Cs-γ6.5 Gy的照射后,与单纯照射组相比,黑茶能明显提高小鼠的外周血白细胞数(white blood cell,WBC)、股骨有核细胞数(the number of bone narrow nucleated cells,BMNC)、骨髓DNA含量以及脾结节数(colony forming unit-spleen,CFU-S),差异具有显著性(P<0.05);小鼠受到137Cs-γ6.0 Gy后,与单纯照射组相比,黑茶可显著提高小鼠肝组织和肺组织中SOD的活力,差异具有显著性(P<0.05);黑茶可使受照(8.0Gy)小鼠存活天数由8.6±1.96 d延长到11.13±2.75 d。提示黑茶有较好辐射防护的作用。This study aimed to investigate the protective effect of Hunan Dark Tea against radiation injuries in mice. Peripheral blood index, immune system index, superoxide dismutase of anti-oxidative index and survival time estimation were used to observe the effect of Dark Tea to protect mice from radiation injuries. Compared with the irradiation-only group ,the results showed that Dark Tea can effectively increase the white blood cell （WBC） ,the number of bone narrow nucleated cells （BMNC）, DNA content of bone marrow and colony forming unit- spleen （CFU-S） of mice irradiated with 6.5 Gy γ-rays. The difference was significant （P 〈 0.05 ）. Besides, the SOD activities of the Liver and lung tissue of mice irradiated with 6.0 Gy 3,-rays in Dark Tea administrated group markedly increased when compared with irradiation-only group. The difference was significant （ P 〈 0.05 ） ; In addition, Dark Tea also prolonged the survival time of mice from 8.6 ± 1.96 d to 11.13 ± 2.75 d after irradiated with 8.0 Gy γ-rays. All these experimental results suggested that Dark Tea have good anti-radiation activities.

关 键 词：黑茶 辐射损伤 抗氧化 抗辐射 

分 类 号：R932[医药卫生—生药学] R961.1[医药卫生—药学]