精氨酸双糖苷对小鼠毒性实验研究  

Study on the Toxicity of Argininyl-Fructoul-Glucose in Mice

作　　者：宋明铭 王佳奇[1] 孙荣花[1] 丁传波[1,2] 刘文丛[1] 郑毅男[1] 

机构地区：[1]吉林农业大学,长春130118 [2]吉林省珲春神州药业有限公司,珲春133300

出　　处：《天然产物研究与开发》2016年第5期786-790,共5页Natural Product Research and Development

基　　金：国家科技部科技型中小企业技术创新基金(13C26212201221);吉林省医药产业发展引导资金专项(20150311050YY)

摘　　要：为观察精氨酸双糖苷(AFG)毒性反应,通过高效阳离子柱洗脱纯化AFG,ICR小鼠80只,随机分为4组,每组20只,雌雄各半。各组分别灌胃给予生理盐水,AFG 30 mg/kg、2 g/kg、6 g/kg,每天给药一次,连续给药90 d,并观察各组小鼠的状态。各组给药结束后,眼球取血,处死,解剖检查,取材、制片和H.E染色进行病理学检查。结果显示,AFG 6 g/kg组,连续给药60 d后,个别小鼠出现眼睛红肿,行动缓慢等现象。AFG低、中、高剂量组连续给药90 d后,与空白对照组比较,各剂量组体重变化和食物消耗量无显著性差异,AFG 6 g/kg组肝、脾指数明显降低(P<0.01,P<0.05);血液生化指标的测定中,AFG 30 mg/kg、2 g/kg组肌酐明显上升(P<0.05),AFG 6 g/kg组谷丙转氨酶、谷草转氨酶升高(P<0.05),甘油三酯明显升高(P<0.01)。其它血液生化指标与空白对照组比较无显著性差异。现有数据表明,90 d内服用AFG对小鼠生理活性和状态无明显毒性作用。The aim of this study was to observe the long-term toxicity of argininyl-fructoul-glucose（AFG） in mice. A total of 80 ICR mice, were randomly divided into 4 groups,20 mice in each group, half male and half female. Each group were lavaged with physiological saline,AFG 30 mg/kg,2 g/kg and 6 g/kg,once a day,last for 90 days,and all animals were daily monitored for abnormalities and mortality. After 90 days,in order to obtain the information on the progression of toxicelogieal changes,mice were fasted for 12h and were subsequently sacrificed by exsanguination followed by a fall,detailed gross necropsy. Embedded tissues were sectioned to approximately 5-6μm in thickness, stained with hematoxylin and eesin（H＆E） before they were examined under a microscope. After administration for 60 days,some mice in AFG 6 g/kg group appeared of eyes redness, slow action and other phenomena. Compared with the control group, no significant differences in weight change and food consumption were detected in AFG 30 mg/kg,2 g/kg and 6 g/kg group. The liver and spleen index of AFG 6 g/kg group decreased significantly（ P 〈 0.01, P 〈 0.05） for serum chemistry analyses, Crea of AFG 30 mg/kg and 2 g/kg groups increased significantly （ P 〈 0.05 ）, the ALT and AST of AFG 6 g/kg group increased （ P 〈 0.05）, TG also rise obviously （P 〈 0.01 ）. Other blood biochemical indexes of sample groups had no significant difference with these of control group. The existing data showed that the 90 d in the use of AFG had no obviously toxicity in mice.

关 键 词：精氨酸双糖苷 ICR小鼠 长期毒性实验 

分 类 号：R285.1[医药卫生—中药学]