多种心脏标志物与冠状动脉钙化积分回归方程的建立  

Establishment of regression equation of multiple cardiac markers and coronary arterial calcification score

作　　者：冯勤颖[1] 黄山[1] 许健[1] 张春阳[2] 陈洁[1] 田禾[1] 刘志琴[3] 王荣品[4] 

机构地区：[1]贵州省人民医院临床检验中心,贵阳550002 [2]贵阳医学院,贵阳550002 [3]贵州省人民医院心内科,贵阳550002 [4]贵州省人民医院放射科,贵阳550002

出　　处：《检验医学与临床》2016年第10期1316-1318,1321,共4页Laboratory Medicine and Clinic

基　　金：贵州省卫生厅科学技术基金资助项目(gzwkj2012-1-062);贵州省省长资金临床应用课题专项研究资助项目[黔省专合字(2012)117号]

摘　　要：目的建立多种心脏标志物与冠状动脉钙化积分的回归方程。方法采用ELISA检测健康人群和冠状动脉不同钙化积分的冠心病患者血清中的白细胞介素-10（IL-10）、妊娠相关血浆蛋白A（PAPP-A）、高迁移率族蛋白B1（HMGB1）、脂联素（APN）、1,25-二羟维生素D3[1,25-（OH）2D3]、缺氧诱导因子-1α（HIF-1α）、白细胞介素-18（IL-18）、基质γ-羧基谷氨酸蛋白（MGP）、骨桥蛋白（OPN）等9种标志物血清水平,并结合性别比和年龄进行统计分析,通过Logistic回归分析建立回归方程,并进行验证。结果各组间IL-10、PAPP-A、1,25-（OH）2D3、HIF-1α、IL-18、MGP、OPN等7种标志物血清水平比较,差异有统计学意义（P〈0.05）;回归方程：经对数转换的钙化积分（Ln P）=5.71＋0.076×年龄＋0.11×HIF-1α-0.50×PAPP-A-0.11×OPN,每组随机抽取6例患者的相关数据进行验算,其钙化积分与实际钙化积分的误差率为0.45%~14.70%。结论多种心脏标志物在不同的钙化积分患者中表达不同,对冠心病有较高的诊断价值,所建立的回归方程具有很好的拟合性,结果可靠,有利于促进心脏标志物的临床应用。Objective To establish the regression equation of multiple cardiac markers and coronary arterial calcification score.Methods The ELISA method was adopted to detect serum interleukin-10（IL-10）,pregnancy associated plasma protein A（PAPP-A）,high mobility group protein B1（HMGB1）,adiponectin（APN）,1,25 dihydroxy vitamin D3[1,25-（OH）2D3],hypoxia induced factor-1α（HIF-1α）,interleukin-18（IL-18）,matrix gamma-carboxy-glutamic acid protein（MGP）and osteopontin（OPN）levels in the healthy population and the patients with different coronary arterial calcification scores of coronary heart disease,moreover which were combined with the sex ratio and age for conducting the statistical analysis,establishing the regression equation by the Logistic regression analysis and performing the verification.Results The serum levels of IL-10,PAPP-A,1,25-（OH）2D3,HIF-1α,IL-18,MGP and OPN had statistical differences among different groups（P0.05）;the regression equation was：Ln P（scores）=5.71＋0.076×AGE＋0.11×HIF-1α-0.50×PAPP-A-0.11×OPN.The related data were randomly extracted from 6patients in each group for conducting the checking calculation,the percentage error of their calcification scores and the actual calcification scores was 0.45%-14.70%.Conclusion The multiple cardiac markers have different expression in the patients with different calcification scores,which has higher diagnostic value for coronary heart disease,the established regression equation has better fitting with reliable results,and is conducive to promote the clinical application of cardiac markers.

关 键 词：心脏标志物 冠心病 冠状动脉钙化结分 回归方程 

分 类 号：R541.404[医药卫生—心血管疾病]