IL-1β对肠上皮细胞屏障通透性的影响  

The effects of IL-1β beta on intestinal epithelial barrier permeability

作　　者：林楠[1] 滕旭[1] 许玲芬[1] 孙梅[1] 

机构地区：[1]中国医科大学附属盛京医院儿科,沈阳110004

出　　处：《国际儿科学杂志》2016年第5期409-412,共4页International Journal of Pediatrics

基　　金：国家自然基金青年科学基金（81400585）;辽宁省自然科学基金（2014021042）;沈阳市科技计划项目（F13-316-1-71）

摘　　要：目的炎症性肠病是儿童和青少年时期重要的慢性胃肠道疾病，肠黏膜屏障的损伤在其发病中起重要作用。本研究通过IL-1β刺激Caco-2细胞单层，体外模拟炎症性肠上皮细胞屏障，为研究炎症性肠病的发病及治疗提供基础。方法体外培养Caco-2细胞21d模拟肠上皮细胞单层屏障。炎症1组从培养第5天开始每隔1天应用IL-1β处理2h检测TEER值，至第2l天。炎症2组于培养第18天换用含IL-1β培养液，分别于处理0、12、24、48、72h检测TEER。正常对照组用普通培养液培养，也于相应时间点检测TEER。结果正常对照组细胞TEER从第5天至第15天逐渐增加，在第15天达到600Ω·cm^2，并到达平台期保持至第21d。炎症1组细胞TEER值均低于正常组细胞，并且直至第21天仍〈500Ω·cm^2。炎症2组细胞显示时间依赖性的TEER逐渐降低，在48h达到最大值，然后在72h轻度回升。结论培养2～3周的Caco-2细胞能够形成具有极性的肠上皮细胞单层，对其给予IL-1β刺激可在体外模拟炎症性肠上皮细胞屏障。Objective Inflammatory bowel disease is an important chronic gastrointestinal disease of childhood and adolescence. Intestinal mucosa barrier damage plays an important role in its pathogenesis. This study attempts to use IL-1β stimulating Caco-2 cell monolayer simulates inflammatory intestinal epithelial barrier in vitro, provides the basis for studying the pathogenesis and treatment of IBD. Methods Caco-2 cells were cul- tivated in vitro until the 21 st day to simulate intestinal epithelial cell monolayer barrier. The cells of inflammation group one were disposed with IL-1β for 2 h since the 5th day and detected TEER every other day until the 21st day. The cells of inflammation group two were disposed with IL-1β at the 18th day for 0,12,24,48,72h, and detected TEER respectively. Normal control group cells were cultured with common medium and detected TEER at the corresponding time point. Results The TEER of Caco-2 cells gradually increased from the 5th to 15th days, reached 600Ω·cm^2 in the 15th day of a plateau until to the 21st day. Since the 5th day,the TEER of inflamma- tion group one were all lower than normal group,and still to the 21st day 〈 500Ω·cm^2. Inflammation group two shows the time dependence TEER gradually reduce,peaked at 48 hours,then a slight increase in 72 hours. Con- dusion The Caco-2 ceils cultured for 2 - 3 weeks can form intestinal epithelial monolayer barrier with polarity, then treated with IL-1β can manufacture inflammatory intestinal epithelial barrier model in vitro.

关 键 词：肠上皮细胞屏障 跨细胞电阻 白细胞介素-1β炎症性肠病 

分 类 号：R725.7[医药卫生—儿科]