5种卤代乙酰胺类消毒副产物对小鼠淋巴瘤细胞Tk基因致突变性研究  

Mutagenic Analysis of Five Haloacetamides Disinfection By-products in the Tk Gene of Mouse Lymphoma Cells

作　　者：袁婷婷[1] 王新亮[2] 董慧峪[3] 

机构地区：[1]北京医院检验科,北京100730 [2]天津市城市规划设计研究院,天津300201 [3]中国科学院生态环境研究中心,北京100085

出　　处：《生态毒理学报》2016年第1期153-158,共6页Asian Journal of Ecotoxicology

基　　金：国家自然科学基金课题(51408590)

摘　　要：卤代乙酰胺类(HAc Am)消毒副产物(DBPs)是饮用水中新兴含氮类DBPs之一。为探究HAc Ams的致突变性,选择小鼠淋巴瘤细胞作为受试细胞,分别考察了一氯代乙酰胺、二氯代乙酰胺、三氯代乙酰胺、一碘代乙酰胺、二碘代乙酰胺(DIAc Ams)共5种HAc Ams对小鼠淋巴瘤细胞Tk基因突变的影响。结果表明,在0.05~20μmol·L^(-1)暴露浓度下,5种HacAms类DBPs对小鼠淋巴瘤细胞呈现出一定的细胞毒性,随着暴露浓度的升高,细胞毒性增强。暴露4 h后,5种HacAms中只有DIAc-Ams呈现出显著的致突变性(暴露剂量高于5μmol·L^(-1)),且以小集落为主。上述研究结果为研究HacAms对哺乳动物细胞的致突变作用提供了基础数据。Haloacetamide（ HAcAm） is one of the emerging nitrogeneous disinfection by-products（ DBPs） in drinking water. To evaluate the mutagenic potential of HAc Am,effects of 5 HAcAms including chloroacetamide,dichloroacetamide,trichloroacetamide,iodoacetamide and diiodoacetamide（ DIAcAm） on thymidine kinase（ Tk）gene in the well-validated mouse lymphoma assay were investigated in this study. The results showed that with the exposure levels from 0. 05 to 20 μmol·L^-1,all the 5 HAcAms investigated exhibited cytotoxicity,which increased with the elevated exposure level. After 4 h of exposure,only DIAc Am increased the frequency of mutant colonies,with a higher proportion of small colonies. These results of HAc Ams provided basic data for the evaluation of muta-genic potential to mammalian cells.

关 键 词：卤代乙酰胺 小鼠淋巴瘤细胞 突变分析 

分 类 号：X171.5[环境科学与工程—环境科学]