促红细胞生成素对大鼠脊髓损伤后神经生长蛋白43和微管相关蛋白-2表达的影响  

Effect of Erythropoietin on GAP-43 and MAP-2 Expression After Spinal Cord Injury in Rats

作　　者：王宁[1] 徐纪伟[1] 刘红敏[1] 华新宇[1] 

机构地区：[1]漯河医学高等专科学校,462002

出　　处：《医学研究杂志》2016年第5期104-107,共4页Journal of Medical Research

基　　金：河南省教育厅自然科学基金资助项目(13B310163)

摘　　要：目的观察促红细胞生成素(erythropoietin,EPO)对大鼠脊髓损伤(spinal cord injury,SCI)的保护作用及神经细胞神经生长蛋白43(GAP-43)、微管相关蛋白-2(MAP-2)表达的影响。方法制作SCI模型,分为假手术组、生理盐水组、EPO治疗组。用免疫组织化学染色方法检测神经细胞内GAP-43和MAP-2的表达变化,同时通过BBB运动评分检测动物的运动功能。结果 EPO治疗组较生理盐水组GAP-43和MAP-2表达水平高,EPO治疗组的BBB运动评分明显优于生理盐水组。结论 EPO能够通过增加GAP-43和MAP-2表达,促进神经细胞生长。Objective To investigate the effect of Erythropoietin on GAP - 43 and MAP - 2 expression after spinal cord injury in rats. Methods Rats were randomly divided into sham group, NS group and erythropoietin treated group. Immunohistochemistry staining was used to observe GAP -43 and MAP -2 expression. BBB test were used to observe motor function in rats. Results Curcumin treatment group compared with NS group, GAP - 43 and MAP - 2 expression were significantly increased. The score of BBB test Erythropoietin treatment group were significantly better than NS group. Conclusion Erythropoietin might promote neurons growth by GAP - 43 and MAP -2.

关 键 词：促红细胞生成素 脊髓损伤 神经细胞神经生长蛋白43 微管相关蛋白-2 

分 类 号：R338[医药卫生—人体生理学]