2513例抗核抗体与抗核抗体谱检测结果对比分析  

Comparative analysis of antinuclear antibody and antinuclear antibody spectrum for 2513 samples

作　　者：蒲泽晏 胥国强[1] 李祥坤[1] 李凤[1] 黄雪梅[1] 

机构地区：[1]遂宁市中心医院检验科,四川遂宁629000

出　　处：《国际检验医学杂志》2016年第10期1346-1347,共2页International Journal of Laboratory Medicine

摘　　要：目的分析本实验室抗核抗体(ANA)与抗核抗体谱(ANAs)15项联合检测的结果,并探讨两者之间的关系。方法分别用间接免疫荧光法(IIF)和免疫印迹法(IBT)检测患者ANA和ANAs,判断其ANA滴度、荧光模型和ANAs结果,并对ANA和ANAs检测结果进行对比分析。结果 471例ANA阳性患者标本中,颗粒型161例,均质型110例,胞质型96例、核仁型37例、着丝点型28例,其他型合计39例;滴度为1∶100者196例,占41.61%(196/471),其中ANAs阳性53例,占27.04%(53/196);滴度为1∶320者123例,占26.11%(123/471),其中ANAs阳性67例,占54.47%(67/123);滴度为1∶1 000者152例,占32.27%(152/471),其中ANAs阳性132例,占86.84%(132/152);2 042例ANA阴性标本中45例ANAs检测阳性,阳性率为2.2%(45/2042);高滴度ANA患者血清中ANAs的阳性率高于低滴度(χ2=123.132,P<0.05)。结论不同滴度的ANA与ANAs之间有一定的关系,如果以ANA进行过筛,阳性再做ANAs会漏检部分患者,建议IIF和IBT联合应用,防止风湿病的漏诊。Objective To analyze the results of combined detection of the 15 indexes of antinuclear antibodies（ANA）and antinuclear antibody spectrum（ANAs）in our laboratory and to investigate the correlation between them.Methods The ANA fluorescence pattern and the ANA titer were detected by the indirect immunofluorescence（IIF）and the ANAs was detected by immunoblotting（IBT）.The results of the ANA and ANAs were analyzed.Results Among the 471 samples of ANA positive,161 cases were speckled pattern,110 cases were homogeneous pattern,96 cases were cytoplasmic pattern,37 cases were nucleolar pattern,28 cases were centromere pattern,and 39 cases were the other pattern.The positive rate of ANA titer of 1∶100was 41.61%（196/471）and positive rate of ANAs was 27.04%（53/196）.The positive rate of ANA titer of 1∶320was 26.11%（123/471）and positive rate of ANAs was 54.47%（67/123）.The positive rate of ANA titer of 1∶1 000 was 32.27%（152/471）and positive rate of ANAs was86.84%（132/152）.45 cases were detected with the ANAs positive of in 2 042 ANA negative samples,the positive rate were 2.2%（45/2 042）.The positive rate of ANAs in high ANA titer samples was higher than that in low ANA titer samples（χ^2=123.132,P〈0.05）.Conclusion The ANA titer and ANAs has certain relationship.If ANA is regarded as positive to test ANAs,a small proportion of patients will miss detection.Thus,the combination detection of IIF and IBT is suggested to avoid the misdiagnosis of rheumatic.

关 键 词：抗核抗体 抗核抗体谱 间接免疫荧光法 免疫印迹法 

分 类 号：R446.6[医药卫生—诊断学]