切断双侧内脏大神经对犬急性坏死性胰腺炎小肠免疫功能的影响  

Effect of Small Intestinal Immunological function after Cutting off the Bilateral Greater Splanchnic Nerve in the Dog with Acute Necrotizing Pancreatitis

作　　者：杨天保[1,2] 孙君军[1] 易芳[1] 杨延辉[1] 

机构地区：[1]河南科技大学第一附属医院,河南洛阳471003 [2]河南科技大学临床医学院,河南洛阳471003

出　　处：《河南科技大学学报（医学版）》2016年第2期87-90,共4页Journal of Henan University of Science & Technology:Medical Science

摘　　要：目的探讨在切断内脏神经后对犬急性坏死性胰腺炎小肠免疫功能的影响。方法选取健康成年杂种犬17只,随机分为假手术组(SO组)5只、急性坏死性胰腺炎模型组(ANP组)6只和ANP+内脏大神经切断组(GSNT组)6只。分别于术前2 h,术后12 h、24 h、3 d、5 d、7 d抽取外周静脉血,检测血浆淀粉酶。7 d后处死动物取小肠黏液检测SIg A浓度,取小肠组织行病理学检查和评分。实验中途死亡动物放弃实验数据,并做死因分析。结果血浆淀粉酶ANP组和GSNT组比较差异无统计学意义(P>0.05);小肠黏液中SIg A浓度,GSNT组高于ANP组但低于SO组,差异有统计学意义(P<0.01);小肠组织病理学评分,GSNT组高于ANP组但低于SO组,差异有统计学意义(P<0.01);ANP组死亡1只。结论切断双侧内脏大神经可改善犬急性ANP时小肠的免疫功能,减轻小肠的病理损伤程度。Objective To research the effect of small intestinal immune after bilateral greater splanchnic nerve transection in dog with acute necrotizing pancreatitis. Methods 17 mongrel heath dogs were randomly divided into three groups,sham operation group （SO group ）5,acute necrotizing pancreatitis model group （ANP group）6,and ANP with bilateral greater splanchnic nerve transection group （GSNT group）6.The plasma levels of pancreatic amylase were monitored at 2 hours before operation.2 hours,12 hours,24 hours,3 days,5 days and 7 days after operation.At post-operation 7 days all animals were killed, small intestinal mucous was collected to detect the level of SIgA,and a section small intestinal was collected to make pathological examination and score.If the animals were dead in process of experiment,all the related data would be abandoned,but the cause of death was investigated. Results There were no significant difference between ANP group and GSNT group on the levels of plasma pancreatic amylase （P 〉0.05 ）.The concentration of SIgA from small intestinal mucous was obvious reducing in ANP group and GSNT group compared with SO group （P 〈0.01 ）,besides it was higher in GSNT group than ANP group（P〈0.01 ）.The pathological scores of small intestinal among the three groups,the ANP group and GSNT group scores were higher than that of SO group （P 〈0.01 ）,while GSNT group was lower than ANP group（P〈0.05）.The ANP group had a dead animal in the experiment. Conclusion Cutting off bilateral greater splanchnic never could improve small intestinal immunological function and alleviate the degree of small intestinal damage.

关 键 词：急性坏死性胰腺炎 内脏大神经 小肠免疫功能 

分 类 号：R657.51[医药卫生—外科学] R392.32[医药卫生—临床医学]