利用超高效液相色谱-高分辨四极杆飞行时间质谱鉴别预混剂中黄霉素  

Indentification of Flavomycin in Premix by Ultra- Performance Liquid Chromatography- Tandem High Resolution Quadrupole Time- of- Flight Mass Spectrometry

作　　者：吴家鑫 刘敏[2] 尚飞[3] 齐鹏 张国栋 梁景乐 

机构地区：[1]中牧实业股份有限公司,农业部兽用生物制品与化学药品重点实验室,北京市兽用多肽疫苗设计与制备工程技术研究中心,北京100095 [2]北京化工大学生命科学与技术学院,北京市生物加工过程重点实验室,北京100029 [3]北京化工大学分析测试中心,北京100029 [4]中牧实业股份有限公司,北京100070

出　　处：《中国兽药杂志》2016年第5期24-28,共5页Chinese Journal of Veterinary Drug

基　　金：国家高技术研究发展计划(2011AA10A214)

摘　　要：利用超高效液相色谱-高分辨四极杆飞行时间质谱对预混剂中的黄霉素5种组分进行鉴别分析。采用C18色谱柱分离,以乙腈-0.1%甲酸水溶液(含2 mmol/L乙酸铵)为流动相梯度洗脱,流速0.4 m L/min,柱温40℃;质谱条件为电喷雾离子源,检测方式为负离子全扫描模式,通过保留时间、精确分子量和二级质谱特征碎片完成对黄霉素的5种组分的鉴别。与高效液相色谱方法比较,本方法具有判断准确、检测快速的特点,具有很好的应用价值。Using ultra- performance liquid chromatography- tandem high resolution quadrupole time- of- flight mass spectrometry,the discriminant analysis of 5 components of flavomycin in premix was conducted. Based on C18 chromatogram column separation, the acetonitrile- 0. 1% formic acid aqueous solution（ including2 mmol / L ammonium acetate） was employed as the mobile phase at flow rate of 0. 4 mL / min for gradient elution at column temperature of 40 ℃; Mass spectrometry conditions were electrospray ionization（ ESI） operated in negative ion full scan mode. The identification of 5 components of flavomycin were implemented based on retention time,exact molecular weight, and secondary mass spectral characteristic fragments. Compared with high performance liquid chromatography,this method is of better application value for its advantages of accurate judgement and rapid detection.

关 键 词：鉴别 黄霉素 超高效液相色谱 高分辨四极杆飞行时间质谱 

分 类 号：R917[医药卫生—药物分析学]