肾间质纤维化实验动物模型的多角度筛选  

Screening the Experimental Animal Model of Renal Interstitial Fibrosis from Multi Angle

作　　者：张红梅[1] 曹旭[2] 王东[3] 金华[3] 刘凯凯[1] 

机构地区：[1]安徽中医药大学中医临床学院,安徽合肥230038 [2]荆门市中医院,湖北荆门448000 [3]安徽中医药大学第一附属医院,安徽合肥230038

出　　处：《贵阳中医学院学报》2016年第3期13-18,共6页Journal of Guiyang University of Chinese Medicine

基　　金：国家自然科学基金项目;项目编号:No:81473673;国家自然科学基金青年科学基金项目;项目编号:No:81403372;安徽高校自然科学研究重点项目;项目编号:No:KJ2016A412

摘　　要：目的:比较腺嘌呤灌胃和单侧输尿管结扎建立大鼠肾间质纤维化模型,从而得到生化、免疫组化及病理指标更符合肾间质纤维化的动物模型,以备后续实验使用。方法:实验大鼠分为正常组、腺嘌呤组和单侧输尿管结扎手术(UUO)组3组,后两组分别运用腺嘌呤灌胃和单侧输尿管结扎建立大鼠肾间质纤维化。观察各组大鼠尿蛋白定量、血肌酐和尿素氮;HE、Masson染色,观察病理变化;免疫组化法检测肾组织E-cadherin、β-catenin及α-SMA的表达。结果:与正常组比较,腺嘌呤组24h尿蛋白、血清尿素氮和血清肌酐均有较为显著升高(P<0.01);UUO组24h尿蛋白有显著性升高(P<0.05)、血清尿素氮和血清肌酐均有较为显著升高(P<0.01)。与腺嘌呤组比较,UUO组24h尿蛋白显著性升高(P<0.05)。与正常组比较,腺嘌呤组、UUO组的E-cadherin平均光密度值有显著性降低(P<0.05),α-SMA和β-catenin平均光密度值有显著性升高(P<0.01、P<0.05);与腺嘌呤组比较,UUO组β-catenin有显著性降低(P<0.05)。病理显示,腺嘌呤组肾小管损伤较UUO组严重。结论:UUO建立的大鼠肾间质纤维化模型在生化、免疫组化及病理指标优于腺嘌呤组。Objective： To compare the models established by adenine irrigation and by unilateral ureteral ligation（ UUO） so as to obtain a better experimental animal model of renal interstitial whose biochemical,im-munohistochemical and pathological indicators meet the standard better. Methods： Experimental rats were divided into normal group,Adenine group and UUO group. The Adenine group and UUO group were given adenine irrigation and unilateral ureteral ligation（ UUO） to establish the experimental animal model of renal interstitial. Observe the urine protein quantitation,Serum creatinine and urea nitrogen,HE,Masson staining,pathological changes,E-cadherin、β-catenin and α-SMA of every group. Results： Compared with the normal group,the 24-h urine protein quantitation,Serum creatinine and urea nitrogen increased in Adenine group（ P 0. 01）,and in UUO group,the urine protein quantitation had a significant increase（ P 0. 05）,while Serum creatinine and urea nitrogen increased as well（ P 0. 01）. Compared with Adenine group,the 24-h urine protein quantitation of UUO group creased remarkably（ P 0. 05）. Compared with the normal group,the average optical density of E-cadherin decreased significantly（ P 0. 05） and Alpha-SMA beta and-catenin average optical density values increased significantly（ P 0. 01,P 0. 05）. Compared with Adenine group,β-catenin in UUO group decreased greatly（ P 0. 05）. Pathologic revealed that renal tubular injury in Adenine group was more serious than that in UUO group. Conclusion： Animal Model of Renal Interstitial Fibrosis established in UUO group is better than Adenine group in biochemistry,immunohistochemistry and pathology.

关 键 词：腺嘌呤 肾间质纤维化 实验研究 

分 类 号：R332[医药卫生—人体生理学]