芪参益气方对心肌梗死治疗作用分子机制的生物信息分析  

Bioinformatics analysis on the treatment mechanisms of Qishen-Yiqi fomular for myocardial infarction

作　　者：林贯川[1] 郑文岭[1] 马文丽[1] 

机构地区：[1]南方医科大学基因工程研究所,广州510515

出　　处：《国际中医中药杂志》2016年第5期436-441,共6页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金（39880032）：广东省领军人才基金（C1030925）

摘　　要：目的运用生物信息学分析工具，从基因的角度阐述芪参益气方对心肌梗死治疗作用的分子机制。方法对基因表达综合数据库（Gene Expression Omnibus，GEO）进行大数据分析，采用酷核组学分析器（Qlucore Omics Explorer,QOE）进行差异基因分析，筛选出治疗作用中的功效基因分子；采用可视化基因注释系统数据库（Database for Annotation，Visualizationand Integrated Discovery online Gene Annotation system，DAVID）进行基因功能富集分析，确定所筛选基因在生物进程中的功能；采用基因／蛋白质相互作用检索数据库（Gene／protein interaction database，STRlNG）进行分析，确定关键基因在治疗过程中分子机制网络图。结果经分析，推论出芪参益气方在对心肌梗死治疗过程中主要上调了包括NFIL3、ARNTL、DBP、FGD4等特殊基因和基因受体基因（NR1D1、NR1D2）的表达，使得生物节律基因、特定结合基因（BHLHE40／41）得到调控。结论基因层面上，芪参益气方可调控细胞再生、炎症抵抗、酶活性等相关基因的表达，使心血管和心脏代谢相关的心肌梗死得到治疗。Objective Based on the age of big data, the treatment mechanisms of Qishen-Yiqi（QSYQ） for myocardial infarction was focused. Methods All the data stemmed from Gene Expression Omnibus （GEO）. For ensuring the efficacy genetic molecular, differentially expressed genes were discobered by Qlucore Omics Explorer （QOE）. And then gene set enrichment analysis was performed by Database for Annotation, Visualization and Integrated Discovery online Gene Annotation system（DAVID） for showing the genes functions during bioprocess. In the end, the predicted genes and proteins interactions networks were demonstrated by Gene/protein interaction database （STRING）. Results The main biological process involved up regulating the expression of some specil genes such as NFIL3, ARNTL, DBP, FGD4 and nuclear receptor genes like NR1D1, NR1D2 while using QSYQ treatment. In such case, BHLHE40/41 was regulated. Conclusion Traditional Chinese medicine, QSYQ, could influence the expression of genes of cytothesis, inflammatory and enzymatic activity as its effect of the molecular mechanism, in order to treat and cure the myocarditis infarction.

关 键 词：芪参益气方 心肌梗塞 分子药理作用机制 生物信息分析 

分 类 号：R259[医药卫生—中西医结合]