IBP通过上皮间质转化促进结肠癌细胞迁移和侵袭  

IBP promotes migration and invasion in colon cancer cells via epithelial to mesenchymal transition

作　　者：何颖[1] 张竹君[2] 

机构地区：[1]第三军医大学新桥医院超声科,重庆400037 [2]第三军医大学西南医院医学检验系临床微生物及免疫学教研室,重庆400038

出　　处：《第三军医大学学报》2016年第11期1281-1285,共5页Journal of Third Military Medical University

基　　金：国家自然科学基金青年项目(81201952)~~

摘　　要：目的探讨干扰素调节因子4结合蛋白(interferon regulatory factor-4 binding protein,IBP)能否影响结肠癌细胞上皮间质转化(epithelial to mesenchymal transition,EMT)的发生,观察IBP在肿瘤细胞增殖、迁移和侵袭中作用。方法干预结肠癌细胞中IBP的表达,构建IBP过表达的HT-29-IBP细胞株与IBP沉默的HCT116-sh IBP细胞株;干预IBP表达后,采用Western blot检测结肠癌细胞EMT相关分子的蛋白表达;Transwell实验检测结肠癌细胞迁移、侵袭能力的改变;MTT实验检测结肠癌细胞增殖能力的改变。结果 HT-29-IBP细胞中上皮相关分子E-cadherin和ZO-1表达水平降低,间质相关分子Fibronectin表达升高,细胞增殖、迁移和侵袭能力增强(P<0.05);HCT116-sh IBP细胞则E-cadherin、ZO-1表达水平升高,Fibronectin及Snail表达降低,细胞增殖、迁移和侵袭能力降低(P<0.05)。结论 IBP通过调节EMT促进结肠癌细胞增殖、迁移和侵袭。Objective To determine the role of interferon regulatory factor-4 binding protein（ IBP）in epithelial to mesenchymal transition（ EMT）,and investigate its effect on the migration and invasion of colon cancer cells. Methods Colon cancer HT-29 and HCT116 cells were transfected with pc DNA3. 1（ ＋）-IBP plasmid or infected with recombined sh IBP lentivirus,and the stable clones were selected with G418 or puromycin. The expression of IBP and EMT-related molecules was detected in the HT-29-IBP cells and HCT116-sh IBP cells by Western blotting. MTT assay was performed to investigate the effect of IBP on the proliferation,and Transwell chamber assay was carried out on the migration and invasion in colon cancer cells.Results Over-expression of IBP decreased the expression levels of epithelial markers,E-cadherin and ZO-1,while increased the mesenchymal marker,Fibronectin,and promoted the proliferation,migration and invasion in the HT-29-IBP cells（ P 〈0. 05）. However,silencing of IBP up-regulated the expression of E-cadherin and ZO-1,but down-regulated the levels of Fibronectin and Snail,and decreased the proliferation,migration and invasion in the HCT116-sh IBP cells（ P 〈0. 05）. Conclusion IBP promotes the proliferation,migration and invasion of colon cancer cells through regulating EMT.

关 键 词：干扰素调节因子4结合蛋白 结肠癌 上皮间质转化 迁移 侵袭 

分 类 号：R341[医药卫生—基础医学] R730.23