姜黄素对肿瘤坏死因子-α刺激的INS-1细胞脂联素受体通路的影响  

EFFECTS OF CURCUMIN ON INS-1 CELL ADIPONECTIN RECEPTOR SIGNALING PATHWAY STIMULATED BY TNF-α

作　　者：高远[1] 白宇娜[2] 韩名宝 宋志新[1] 雷婷[1] 闻颖[1] 

机构地区：[1]哈尔滨医科大学营养与食品卫生学教研室,哈尔滨150081 [2]哈尔滨市疾病预防控制中心,哈尔滨150056

出　　处：《营养学报》2016年第2期181-185,共5页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30771805);国家自然科学基金(No.81172650)

摘　　要：目的研究姜黄素(curcumin,CUR)对肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)刺激的INS-1细胞功能损害的影响及可能的作用机制。方法培养INS-1大鼠胰岛瘤细胞,不同浓度(0、0.3125、0.625、1.25、2.5、5、10、20、40、80、160μmol/L)CUR作用24 h后,用MTT方法检测生长状态,用显微镜观察细胞形态。Real-time PCR法测定CUR对TNF-α刺激的INS-1细胞胰岛素m RNA表达的影响。ELISA法测定CUR对TNF-α刺激的INS-1细胞胰岛素分泌的影响。Western Blot法检测CUR对TNF-α刺激的INS-1细胞脂联素受体1(adiponectin receptor-1,Adipo R1)和葡萄糖转运蛋白2(glucose transporter-2,Glut2)蛋白的表达。结果 20μmol/L以下浓度的CUR对INS-1细胞无毒性作用,对INS-1细胞形态无影响。CUR改善了TNF-α刺激的INS-1细胞胰岛素m RNA表达和胰岛素分泌减少,在CUR浓度为10μmol/L时作用效果最为显著(P<0.05)。CUR改善了TNF-α刺激的INS-1细胞Adipo R1、Glut2蛋白表达降低(P<0.05)。结论 CUR可改善TNF-α对INS-1细胞胰岛素合成和分泌的抑制作用,其作用机制可能与脂联素受体通路有关。Objective To investigate the effect of curcumin（CUR） on pancreatic beta–cell line INS-1 stimulated by tumor necrosis factor-α（TNF-α） and the possible mechanism. Methods INS-1 cells were cultured with different concentrations of CUR（0,0.3125,0.625, 1.25,2.55,10,20,40,80,160 μmol/L） for 24 h. The cell viability was determined by MTT assay and the cell morphological changes were observed under microscope. The expression of insulin m RNA and insulin secretion were measured by real-time PCR and ELISA respectively, and the protein expression of adiponectin receptor-1（Adipo R1） and glucose transporter-2（Glut2） were measured by Western blot. Results CUR at the concentration lower than 20 μmol/L had no toxicity on INS-1, nor did it change the cell morphology. In addition, CUR improved the decreased expression of insulin m RNA and insulin secretion in INS-1 cells treated by TNF-α, especially at CUR concentration of 10 μmol/L（P〈0.05）. Moreover, CUR improved the protein expression of Adipo R1 and Glut2（P〈0.05）. Conclusion CUR could improve the decreased insulin secretion and insulin m RNA expression in INS-1 cells induced by TNF-α, and the effects may be related to the adiponectin receptor pathway.

关 键 词：INS-1细胞 肿瘤坏死因子-Α 姜黄素 脂联素受体 

分 类 号：R285.5[医药卫生—中药学]