白藜芦醇对K562细胞增殖凋亡及分化的影响  

EFFECTS OF RESVERATROL ON PROLIFERATION APOPTOSIS AND DIFFERENTIATION OF K562 LEUKEMIA CELLS

作　　者：朱慧丽[1] 聂鸿靖[1] 邓炳楠[1] 段瑞峰[1] 金宏[1] 陈照立[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《营养学报》2016年第2期186-190,共5页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.81471812)

摘　　要：目的研究白藜芦醇(resveratrol,Res)对人K562细胞增殖、凋亡、分化及血红蛋白合成的影响。方法通过细胞增殖-毒性检测试剂盒(CCK-8)法检测Res对K562细胞增殖的影响,流式细胞分析仪检测Res对K562细胞凋亡率的影响,联苯胺染色及血红蛋白测定检测Res对K562细胞红系分化及血红蛋白合成的作用。结果不同浓度Res作用后可时间和剂量依赖性地抑制K562细胞的增殖,可浓度依赖性地促进K562细胞的凋亡;Res单独作用后未能引起K562细胞联苯胺染色阳性率和血红蛋白含量发生变化。而Res预处理后能增加氯化血红素诱导的细胞联苯胺染色阳性率和血红蛋白含量。结论白藜芦醇能够抑制K562细胞增殖、促进其凋亡,白藜芦醇可增强K562细胞红系分化的能力,但并不能直接引起K562细胞的红系分化。Objective To determine the effects of resveratrol（Res） on proliferation, apoptosis, differentiation and hemoglobin synthesis of K562 leukemia cells. Methods The effect of Res on the proliferation of K562 cells was detected by CCK-8 assay. The apoptosis rates of K562 cells induced by Res were analyzed by flow cytometry. The erythroid differentiation and hemoglobin synthesis of K562 cells were determined by benzidines staining and hemoglobin synthesis test. Result Res could inhibit the proliferation of K562 cells in a dose and time-dependent manner, and promote the apoptosis of K562 cells in a dose-dependent manner. Treatment of K562 cells with only Res did not induce the change of positive staining rate of K562 cells and hemoglobin content. However, pre-treatment with Res could increase the positive staining rate and hemoglobin content of the cells induced by hemin. Conclusion Res can inhibit the proliferation of K562 cells and induce its apopotosis. Res can also promote the erythroid differentiation of K562 cells, but cannot directly induce erythroid differentiation of K562 cells.

关 键 词：白藜芦醇 K562细胞 增殖 凋亡 分化 

分 类 号：R285[医药卫生—中药学]