化学-物理法制备多孔纳米羟基磷灰石/Ⅰ型胶原/聚乙烯醇复合水凝胶及其性能  

Preparation and Performance of Porous Nano-hydroxyapatite/Ⅰ-type Collagen/Polyvinyl Alcohol Composite Hydrogel via a Chem-Physical Method

作　　者：周莉[1] 王泽巩 罗仲宽[1] 梁翔禹[1] 

机构地区：[1]深圳大学化学与化工学院,深圳518060

出　　处：《材料导报》2016年第8期41-45,68,共6页Materials Reports

基　　金：深圳市战略性新兴产业发展专项资金资助项目(JSGG20120614164013545;NYSW20130329010039)

摘　　要：以纳米羟基磷灰石(n-HA)、Ⅰ型胶原(Ⅰ-Col)、聚乙烯醇(PVA)为原料,采用化学-物理交联法制备人工角膜支架材料。通过对比测试其含水率、孔隙率、力学拉伸性能、扫描电镜及红外光谱,探讨了戊二醛加入浓度和冷冻解冻次数对材料性能的影响。结果表明当m(PVA)∶m(n-HA+Ⅰ-Col)=7∶1,戊二醛浓度为0.04mol/L时,复合水凝胶材料具有较好的综合性能:含水率为70.41%;孔隙率为58.95%;拉伸强度为3.87 MPa;红外分析表明原材料与交联剂之间产生了交联反应;扫描电镜分析表明经冷冻解冻物理交联5次,该复合水凝胶呈均匀三维多孔结构;生物相容性实验测得兔角膜上皮细胞增殖良好,毒性为Ⅰ级,达到国际要求,属于合格医用材料。Abstract Artificial cornea scaffolds were prepared via the chem-physical method by using nano-hydroxyapatite （n-HA）, I-type collagen （ I-Col）, polyvinyl alcohol （PVA）. The effects of different freezing-thawing cycles, the mass fraction of glutaraldehyde content on the performance of the composite material were studied by testing its moisture content, porosity, tensile strength, SEM and FT-IR. The experimental results showed that when the mass ratio of PVA and I-Col＋ I -HA was 7 ： 1, and the concentration of GA was 0. 04 mol/L, the composite hydrogel material had an excellent integrated performance. The water uptake of hydrogel composites was about 70. 41% （mass frac- tion）. The porosity in hydrogel composites was about 58. 95%. The tensile strength was 3. 87 MPa. FT-IR showed that there was a cross-linking reaction between components. The composite hydrogel presented three-dimensional porous structure with 5 freezing-thawing cycles under SEM. cells cytotoxic of the hydrogel composites by MTT assay, Corneal epithelial cells of the rabbits were used to test the and the results confirmed that it had good biocompatibility and belonged to qualified medical biological materials.

关 键 词：胶原 羟基磷灰石 聚乙烯醇 化学物理法 人工角膜支架 

分 类 号：O648[理学—物理化学]