棘腹蛙TNFα基因的克隆与序列分析  

Molecular cloning and analysis of Tumor Necrosis Factor alpha(TNFα) from Paa boulengeri

作　　者：罗洁[1] 贺明淑 袁万安[1] 樊汶樵[1] 杨帆[1] 孙翰昌[1] 徐敬明[1] 

机构地区：[1]重庆文理学院林学与生命科学学院重庆珍稀濒危水产资源保护与开发研究中心,重庆永川402168 [2]重庆电信职业学院,重庆永川402160

出　　处：《中国兽医杂志》2016年第4期9-12,I0002,共5页Chinese Journal of Veterinary Medicine

基　　金：重庆市科技计划重点专项(cstc2015jcyj BX0013);重庆市科技计划项目(cstc2014jcyj A80042);重庆市教委科学技术研究项目(KJ1401105);重庆文理学院人才引进项目(R2013LS13;R2014LX07)

摘　　要：为了解棘腹蛙TNFα基因的相关信息,对棘腹蛙的TNFα基因进行了克隆和序列分析。克隆了全长为936 bp、ORF长度为675 bp的TNFα-Pb基因;对该基因的蛋白质结构分析显示,TNFα-Pb的信号肽由44个氨基酸组成,随后由2个α螺旋,和10个β折叠形成小分子蛋白;系统进化分析发现,TNFα-Pb与两栖类聚为一枝,与高等哺乳动物存在功能分化的可能。本研究为了解棘腹蛙的生物反应调节机制奠定了一定基础。Tumor necrosis factor alpha（TNFα）is a cytokine involved in immune regulation,apoptosis,and inflammation pathways.Here,we studied the sequence feature,protein structure and phylogenic characteristic of TNFα from Paa boulengeri,The results showed that,TNFd-pb gene was 936 bp in length and the protein structure of TNFα-Pb was a small molecular protein that consisted of 44 amino acid length of signal peptide,two alpha helixes and ten beta sheets.And the phylogenic analysis showed that TNFα-Pb was in the cluster with other amphibian but may have different functions when compared with mammals.Hence,this study may lay the foundation to reveal the immune-regulation mechanism of Paa boulengeri in future.

关 键 词：棘腹蛙 肿瘤坏死因子Α 克隆 序列分析 

分 类 号：R394.2[医药卫生—医学遗传学]