猪口蹄疫O型合成肽VP1结构蛋白ELISA抗体检测方法的建立  

Development of an ELISA based on synthetic peptide for detection of porcine foot-and-mouth disease virus VP1 antibody infection by O serotype

作　　者：董春娜 肖进[1,2] 张蕾 巴利民 栗利芳 宋芳 齐鹏 

机构地区：[1]农业部兽用生物制品与化药重点实验室北京市兽用多肽疫苗设计与制备工程技术中心中牧实业股份有限公司研究院,北京丰台100070 [2]中国农业大学动物医学院,北京海淀100093

出　　处：《中国兽医杂志》2016年第4期39-42,共4页Chinese Journal of Veterinary Medicine

基　　金："十二五"863项目牛口蹄疫合成肽疫苗研制(2011-AA10A211)

摘　　要：根据猪口蹄疫O型流行毒株VP1序列设计出5条合成肽,以固相法合成并以此合成肽作为包被抗原建立猪口蹄疫O型合成肽VP1结构蛋白ELISA抗体检测方法,并对该方法敏感性、特异性、重复性等进行验证。结果表明:该检测方法敏感性为96.0%,特异性为99.1%,批内与批间重复试验变异系数小于10.0%。比对试验结果显示,该检测方法与UBI猪口蹄疫病毒VP1结构蛋白抗体酶联免疫吸附试验诊断试剂盒符合率为93.2%,与中国农业科学院兰州兽医研究所口蹄疫O型液相阻断ELISA抗体检测试剂盒符合率为85.7%。该猪口蹄疫O型合成肽VP1结构蛋白ELISA抗体检测方法敏感性好、特异性强、稳定性高、操作简便,可用于检测O型口蹄疫抗体水平。Five specific peptides of FMDV VP1 were synthesized by a solid-phase method and used to detect antibody of FM-DV serotype O.The ELISA reaction system was optimized and the sensitivity,specificity and repeatability were tested.The resultshowed that the sensitivity and specificity of this method were 96.0% and 99.1%,and variations of intra-assay and inter-assay re-peatability test of the method were below 10.0%.Comparing this assay with UBI VP1 ELISA kits and the liquid phase blockingELISA kits by detecting 280 samples,we showed that the agreement was 93.2% and 85.7%,respectively.This assay has high sen-sitivity and specificity,and is also stable and convenient,which can be used to monitor the antibody level of FMDV serotype O.

关 键 词：口蹄疫 合成肽 抗体 ELISA 

分 类 号：S854.43[农业科学—临床兽医学]