PrP蛋白缺失小鼠单胺能神经递质及受体mR-NA表达量的变化  

Changes of mRNA Expression Quantity of Monoamine Neurotransmitters and Their Receptors in Pr P Deficient Mice

作　　者：王欣宇[1,2] 鞠传静[3] 孟轲音[2] 刘文森[2] 郞需龙 曲桂娟[1] 万家余[2] 

机构地区：[1]吉林农业大学动物科学技术学院,长春130118 [2]军事医学科学院军事兽医研究所,长春130122 [3]吉林大学第四医院一汽总医院,长春130011

出　　处：《吉林农业大学学报》2016年第2期194-197,202,共5页Journal of Jilin Agricultural University

基　　金：吉林省自然科学基金项目(20140101027JC;20130101086JC);国家自然科学基金项目(31201714)

摘　　要：以C57BL/6野生鼠及Pr Pc蛋白敲除小鼠为研究对象,采用荧光定量PCR方法研究多巴胺(DA)、5-羟色胺及其特异性受体的mRNA表达量在脑中的变化规律。结果表明:Pr Pc蛋白缺失小鼠脑中5-HTR-1A,5-HTR-1B,5-HTR-2A,5-HTR-2C,DA显著高于野生鼠;5-HT,DR1,DR2,DR3,DR4显著低于野生鼠。说明Pr Pc蛋白对中枢神经系统有着重要的调节作用。The purpose of this study was to identify the changing laws of differential expressions of Dopamine（ DA）,seroton（ 5-HT） mRNAs between cerebrum of Pr Pcdeficient mice and C57 BL / 6wild mice by quantitative real-time PCR. Compared with cerebrum of C57 BL / 6 wild mice,5-HTR-1A,5-HTR-1B,5-HTR-2A,5-HTR-2C and DA of cerebrum of Pr Pcdeficient mice were significantly higher,and 5-HT,DR1,DR2,DR3 and DR4 were significantly lower. The results demonstrate that Pr Pcprotein plays an important role in regulating central nervous system.

关 键 词：朊蛋白 神经递质 受体 

分 类 号：S858.91[农业科学—临床兽医学] S865.13[农业科学—兽医学]