丙酸睾酮对小鼠睾丸NT-3和NT-4表达的影响  

Effects of Testosterone Propionate on Expression of NT-3 and NT-4 of Mice

作　　者：李洪蛟[1] 陈树雄[1] 蒋彦文 巴桑旺堆[2] 朱彦宾[3] 李纯锦[1] 周虚[1] 

机构地区：[1]吉林大学动物科学学院,长春130062 [2]西藏农牧科学院,拉萨850000 [3]西藏农牧科学院畜牧兽医研究所,拉萨850009

出　　处：《吉林农业大学学报》2016年第2期203-207,共5页Journal of Jilin Agricultural University

基　　金：国家自然科学基金项目(31301224)

摘　　要：以30日龄雄性小鼠为研究对象,用丙酸睾酮分别处理小鼠6,12,24 h后收集睾丸,提取睾丸总RNA和总蛋白,通过实时荧光定量和Western-Blot方法探究了雄激素对睾丸中神经营养因子NT-3及NT-4表达的影响。荧光定量结果表明:丙酸睾酮处理小鼠,12 h组和24 h组NT-3及NT-4的表达显著升高,其他各组间无显著差异。Western-Blot结果与荧光定量结果一致。说明丙酸睾酮能够调节小鼠睾丸中NT-3和NT-4的表达。Studies showed that neurotrophic factors and androgen play an important role in regulating animal reproduction. This study took 30-day-old mice as research object,treating them with testosterone propionate intraperitoneally. And testes were collected at 6,12 and 24 h after injection respectively. Total RNA and total protein were extracted for quantitative analysis to explore the effects of androgen on testicular expression of neurotrophic factors NT-3 and NT-4 by RT-PCR and Western-Blot. The results of RT- PCR indicate that the expression of NT-3 and NT-4 in testes of mice treated with testosterone propionate has significantly increased at 12 h and 24 h groups,while for other groups significant differences have not been found. The results of Western-Blot show an accordant trend with that of RT-PCR,suggesting that testosterone propionate may regulate the expression of NT-3 and NT-4 in testes of mice.

关 键 词：丙酸睾酮 小鼠 神经营养因子 神经营养素-3 神经营养素-4 

分 类 号：S814.1[农业科学—畜牧学] S865.13[农业科学—畜牧兽医]