体外反搏对急性心肌梗死患者血浆微小RNA-126表达量的影响  

Effect of enhanced external counterpulsation on the expression of microRNA-126 in patients with acute myocardial infarction

作　　者：张建超[1] 褚现明[2] 徐昌[3] 李丹[2] 高学正 安毅[2] 

机构地区：[1]青岛大学医学院,山东青岛266000 [2]青岛大学附属医院心内科,山东青岛266000 [3]青岛大学附属医院肿瘤放疗科,山东青岛266000

出　　处：《岭南心血管病杂志》2016年第2期123-126,148,共5页South China Journal of Cardiovascular Diseases

基　　金：国家自然基金(项目编号:81471546);山东省卫计委科技发展计划项目(项目编号:2014WS0173);青岛市博士后应用研究项目(项目编号:2014-12)

摘　　要：目的通过增强型体外反搏治疗急性心肌梗死(acute myocardial infarction,AMI)患者,观察其对血浆微小RNA-126(miR-126)表达量的影响。方法将116例已行经皮冠状动脉介入(percutaneous coronary intervention,PCI)治疗的AMI患者按随机数字表法分为两组:标准治疗组56例,给予标准药物治疗;反搏治疗组60例,除标准药物治疗外,于PCI治疗后1周即予以36 h(每天1 h、每周治疗6d、连续6周)的反搏治疗。另外,选择20名门诊健康体检者作为健康对照组。采用实时荧光定量聚合酶链反应(polymerase chain reaction,PCR)法测定PCI治疗后1 d、2周、7周后miR-126血浆表达量,同时全程记录患者心绞痛的发作情况。结果与健康对照组比较,AMI患者血浆miR-126表达量明显降低,差异有统计学有意义(P<0.05)。反搏治疗组血浆miR-126表达量在反搏治疗前与标准治疗组比较,差异无统计学意义(P>0.05);反搏治疗后血浆miR-126表达量明显高于标准治疗组,差异有统计学意义(P<0.05);并且疗效优于标准治疗组,差异有统计学意义(P<0.05)。结论增强型体外反搏促进miR-126的表达,血浆miR-126表达量将成为评价心肌梗死患者预后的新标志物。Objectives To observe the plasma miR-126 expression level in patients with acute myocardial infarction （AMI） after enhanced external counterpulsation （EECP） therapy. Methods Totally 116 AMI patients who accepted percutaneous coronary intervention （PCI） were randomly assigned into 2 groups： conventional treatment group （group A, n=56） and EECP group （group B, n=60）. Patients in both groups received conventional treatment for 6 weeks, and patients in group B received additional EECP treatment for 36 hours （one hour every day） one week after PCI. Another 20 healthy persons were collected as control group. Real-time polymerase chain reaction （PCR） was adopted to detected plasma miR-126 expression levels 1 d, 2 weeks and 7 weeks after PCI. At the same time, we recorded angina attacks in all the patients. Results Compared with healthy controls, plasma miR-126 expression level decreased in patients with AMI obviously （P〈0.05）. There was no difference in plasma miR-126 expression level between group A and group B before EECP therapy （P〉0.05）. After a period of treatment with EECP, plasma miR-126 expression level in group B was significantly higher than that in group A （P〈0.05）, and the therapy effect was significantly better in group B than in group A （P〈0.05）. Conclusions EECP promotes the expression of miR-126, and plasma miR-126 expression level can become a new marker for evaluating the prognosis of patients with AMI.

关 键 词：心肌梗死 反搏动术 血管成形术 经腔 经皮冠状动脉 miRNA-126 

分 类 号：R542.22[医药卫生—心血管疾病]