反式脂肪酸摄取加重糖尿病小鼠脂代谢异常及初步机制研究  

Trans Fatty Acids Aggravate Lipid Metabolism Dysfunction in STZ-induced Diabetic Mice and its Possible Mechanism

作　　者：赵小娜[1] 王聪[1] 申程[1] 朱洪[1] 韩莎莎[2] 董震[3] 孙爱军[1] 葛均波[1] 

机构地区：[1]复旦大学附属中山医院上海市心血管病研究所,上海市200032 [2]山东大学心内科,山东省济南市250012 [3]复旦大学附属华山医院心内科,上海市200032

出　　处：《中国分子心脏病学杂志》2016年第2期1659-1663,共5页Molecular Cardiology of China

摘　　要：目的探究反式脂肪酸摄取在糖尿病小鼠模型中对脂代谢的影响及初步机制研究。方法成年C57BL/7雄性小鼠随机分为四组,分别为正常饮食组(ND),链脲霉素皮下注射后正常饮食组(STZ+ND),反式脂肪酸饮食组(TD),链脲霉素皮下注射后反式脂肪酸饮食组(STZ+TD),喂养16周后小动物超声检测心功能,安乐死后比较各组间脂肪含量,血浆中甘油三脂(TG),总胆固醇(TC)的水平和心肌胶原含量的变化;透射电镜观察心肌组织中脂滴和线粒体数量和形态的差异;蛋白质印记检测心肌组织中长链脂酰辅酶A合成酶1(ACSL1)和脂肪甘油三脂酯酶(ATL)的表达水平变化。结果四组小鼠心功能正常(P>0.05)。与STZ+ND组相比,STZ+TD组血清TG升高,棕色脂肪体重比下降,心肌组织脂滴数增加(P<0.05)。与ND组和TD组相比,STZ+TD心肌ACSL1表达增加(P<0.05)。结论反式脂肪酸加重STZ诱导的糖尿病小鼠脂代谢异常,这一作用可能是通过调节ACSL1的表达来实现的。Objective To assess the effect of trans fatty acid on lipid metabolism in STZ-induced diabetic mice,and to reveal the possible mechanism.Methods Adult C57BL/6 male mice were randomly divided into four groups：normal diet group（ND）,normal diet after strcptozotocin subcutaneous injection group（STZ＋ND）.trans fatty acids diet group（TD）,trans fatty acids diet after streptozotocin subcutaneous injection group（STZ＋TD）.The cardiac function was tested after 16 weeks diet,then mice were sacrificed.Adipose weight,plasma lipid concentrations and myocardial collagen content were measured.Lipid droplets and mitochondria were assessed by transmission electron microscope.Long chain acyl-CoA synthetase l（ACSLl） and adipose triglyceride lipase（ATL） that regulate lipid metabolism were tested by western blot.Results Cardiac function of these four groups were normal（P 〈0.05）.Compared with STZ＋ND group,the plasma TG was higher,brown fat weight decreased and the lipid droplets were increased in STZ＋TD group（P 〈0.05）.The expression of ACSL1 in STZ＋TD group was much higher than ND group and TD group（P 〈0.05）.Conclusions trans fatty acid can accelerate the lipid metabolism dysfunction in STZ-induccd diabetic mice.This influence may be implicated through regulating the expression of ACSL1.

关 键 词：反式脂肪酸 糖尿病 长链脂酰辅酶A合成酶1 甘油三酯 

分 类 号：R587.2[医药卫生—内分泌] R589.2[医药卫生—内科学]