ANAC005 is a membrane-associated transcription factor and regulates vascular development in Arabidopsis  

作　　者：Jun Zhao Jiang-Shu Liu Fu-Ning Meng Zhen-Zhen Zhang Hao Long Wen-Hui Lin Xiao-Min Luo Zhi-Yong Wang Sheng-Wei Zhu 

机构地区：[1]Key Laboratory of Plant Molecular Physiology,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Institute of Molecular Cell Biology,College of Life Science,Hebei Normal University,Shijiazhuang 050016,China [4]School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Stanford,California 94305,USA [5]Department of Plant Biology,Carnegie Institution for Science,Shanghai 200240,China

出　　处：《Journal of Integrative Plant Biology》2016年第5期442-451,共10页植物学报（英文版）

基　　金：supported by the National High Technology Research and Development Program of China(2012AA101108);National Natural Science Foundation of China(31171614);Ministry of Agriculture of China(2014ZX08001)

摘　　要：Vascular tissues are very important for providing both mechanical strength and long-distance transport. The molecular mechanisms of regulation of vascular tissue develop- ment are still not fully understood. In this study we identified ANACoo5 as a membrane-associated NAC family transcription factor that regulates vascular tissue development. Reporter gene assays showed that ANACoo5 was expressed mainly in the vascular tissues, increased expression of ANACoo5 protein in transgenic Arabidopsis caused dwarf phenotype, reduced xylem differentiation, decreased lignin content, repression of a lignin biosynthetic gene and genes related to cambium and primary wall, but activation of genes related to the secondary wall. Expression of a dominant repressor fusion of ANACoo5 had overall the opposite effects on vascular tissue differ- entiation and lignin synthetic gene expression. The ANACoo5- GFP fusion protein was localized at the plasma membrane, whereas deletion of the last 20 amino acids, which are mostly basic, caused its nuclear localization. These results indicate that ANACoo5 is a cell membrane-associated transcription factor that inhibits xylem tissue development in Arabidopsis.Vascular tissues are very important for providing both mechanical strength and long-distance transport. The molecular mechanisms of regulation of vascular tissue develop- ment are still not fully understood. In this study we identified ANACoo5 as a membrane-associated NAC family transcription factor that regulates vascular tissue development. Reporter gene assays showed that ANACoo5 was expressed mainly in the vascular tissues, increased expression of ANACoo5 protein in transgenic Arabidopsis caused dwarf phenotype, reduced xylem differentiation, decreased lignin content, repression of a lignin biosynthetic gene and genes related to cambium and primary wall, but activation of genes related to the secondary wall. Expression of a dominant repressor fusion of ANACoo5 had overall the opposite effects on vascular tissue differ- entiation and lignin synthetic gene expression. The ANACoo5- GFP fusion protein was localized at the plasma membrane, whereas deletion of the last 20 amino acids, which are mostly basic, caused its nuclear localization. These results indicate that ANACoo5 is a cell membrane-associated transcription factor that inhibits xylem tissue development in Arabidopsis.

关 键 词：ANACoo5 ARABIDOPSIS NAC vascular tissues development 

分 类 号：Q943.2[生物学—植物学]