一测多评法测定银杏叶中总内酯含量  

Determination of Total Lactones Content in Ginkgo Folium by QAMS

作　　者：黄荣妹 刘伟锐[2,3] 刘翠红[2,3] 钟鸣[2,3] 

机构地区：[1]广州市荔湾区龙津街社区卫生服务中心,广东广州510170 [2]广州白云山汉方现代药业有限公司,广东广州510240 [3]中药提取分离过程现代化国家工程研究中心,广东广州510970

出　　处：《今日药学》2016年第4期231-234,共4页Pharmacy Today

基　　金：广州市科技计划项目(201503020011)

摘　　要：目的建立测定银杏叶中总内酯含量的一测多评的方法。方法以白果内酯为指标,建立银杏内酯A、银杏内酯B、银杏内酯C与白果内酯之间的相对校正因子,以样品中测出的白果内酯含量为参照,分别计算出银杏内酯A、银杏内酯B、银杏内酯C的含量,进而得出总内酯的含量,从而建立起仅以白果内酯为对照品的一测多评法,并通过与外标法实测值比较验证。结果建立了银杏叶中总内酯的一测多评法,10批样品的计算结果与外标法的测定值之间没有显著差异。结论同时测定银杏叶中白果内酯、银杏内酯A、银杏内酯B、银杏内酯C等4种成分的一测多评法是准确可靠的,该方法可以用与银杏叶总内酯成分的定量分析。OBJECTIVE To establish the QAMS（ quantitative analysis of multi-components by single-marker） method for the simultaneous determination of the total lactones content in Ginkgo Folium. METHODS The bilobalide was used as index,and the relative correlation factors（ RCF） of ginkgolides A,B,C to bilobalide were determined by HPLC-ELSD. The content of bilobalide was determined firstly,and the contents of ginkgolides A,B,C were determined by the relative correlation factors with the content of bilobalide. The content of total lactones was determined by bilobalide and ginkgolides A,B,C. The quantitative analysis of multicomponents by single-marker with bilobalide as the marker was established,and verified by comparing with the external standard method. RESULTS The method used to determine the content of total lactones was established and there was no significant difference between the calculated contents and the contents determined by external standard method in those 10 batches of samples.CONCLUSION The QAMS method for the simultaneous determination of the total lactones content in Ginkgo Folium is accurate and feasible,and can be applied for the quality control of Ginkgo Folium.

关 键 词：一测多评法 相对校正因子 银杏叶 总内酯 

分 类 号：R284[医药卫生—中药学]